概述

  • 产品名称Anti-Hemogen抗体
    参阅全部 Hemogen 一抗
  • 描述
    兔多克隆抗体to Hemogen
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Hemogen.

    (Peptide available as ab66549.)

  • 阳性对照
    • This antibody gave a positive signal in the following Human Lysates: Testis Tissue, Ovary Tissue, Pancreas Tissue, Lymph node Tissue, Brain Tissue (data not shown), Spleen Tissue (data not shown), Thymus Tissue (data not shown), HeLa Whole Cell (data not shown), SW480 Whole Cell (data not shown), HepG2 Whole Cell (data not shown)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab66550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 55 kDa).

靶标

  • 功能Regulates the proliferation and differentiation of hematopoietic cells. Overexpression block the TPA-induced megakaryocytic differentiation in the K562 cell model. May also prevent cell apoptosis through the activation of the nuclear factor-kappa B (NF-kB).
  • 组织特异性Expressed in hematopoietic precursor cells (at protein level). Detected in CD34+ and K562 leukemia cell line (at protein level). Expressed in bone marrow, testis, thymus. Also expressed in thymus and thyroid tumors, non-Hodgkin lymphoma, various leukemia cell lines, peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) of patients with leukemia.
  • 发展阶段Expressed in fetal liver, kidney and brain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EDAG antibody
    • EDAG-1 antibody
    • Erythroid differentiation-associated gene protein antibody
    • Hemgn antibody
    • HEMGN_HUMAN antibody
    • Hemogen antibody
    • Hemopoietic gene protein antibody
    • Negative differentiation regulator protein antibody
    see all

Anti-Hemogen antibody 图像

  • All lanes : Anti-Hemogen antibody (ab66550) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Human ovary tissue lysate - total protein (ab30222)
    Lane 3 : Human pancreas tissue lysate - total protein (ab29816)
    Lane 4 : Human lymph node tissue lysate - total protein (ab29871)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

Anti-Hemogen antibody (ab66550)参考文献

ab66550 has not yet been referenced specifically in any publications.

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