Hemin Assay试剂盒(ab65332)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
概述
-
产品名称
Hemin Assay试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
检测类型
Quantitative -
检测时间
0h 30m -
产品概述
Hemin Assay Kit (ab65332) utilizes peroxidase activity in the presence of hemin to provide a simple, exquisitely sensitive assay which causes the conversion of a colorless probe to a strongly colored (λ = 570) compound. Trace amounts of Hemin can be quantitated in the 5-160 pg (10-250 fmol) range.
Visit our FAQs page for tips and troubleshooting. -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K672 Heme Colorimetric Assay Kit. K672-100 is the same size as the 100 test size of ab65332.
Free hemin results from the breakdown of hemin-containing proteins such as hemoglobin and myoglobin. It can be detected in various body fluids such as saliva, urine and csf under various pathological states. Free hemin exists in cells at a very minute concentrations (< 1µM ˜ 650 ng/ml) exerting regulatory functions such as repression of nonspecific d-aminolevulinate synthase expression and induction of microsomal hemin oxygenase-1. Hemin can stimulate growth of oral bacteria involved in gingivitis and is an indicator of possible pathological conditions when found in the urine or feces.
-
平台
Microplate reader
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 100 tests 2000 tests Apoperoxidase Enzyme Mix 1 vial 20 vials Assay Buffer V 1 x 25ml 20 x 25ml OxiRed Probe II 1 x 0.2ml 20 x 0.2ml Hemin Standard 1 vial 20 vials Substrate V 1 x 1ml 20 x 1ml -
研究领域
-
相关性
Free hemin results from the breakdown of hemin-containing proteins such as hemoglobin and myoglobin. Hemin level has been used as an indicator of possible pathological conditions when found in the urine or faeces. It plays regulatory functions for enzyme activities.
图片
-
Uraskai et al investigates the effect of perturbations to uridine homeostasis on heme-deficiency response pathway. Mouse liver hemin concentration was measured using ab65332.
-
Hemin measured in various biofluids showing concentration (nM)
-
A: Time course of development of color and B: Standard Curve at 30 minutes for Hemin standard.
数据表及文件
-
SDS download
-
Datasheet download
文献 (17)
ab65332 被引用在 17 文献中.
- Chandana M et al. Malaria parasite heme biosynthesis promotes and griseofulvin protects against cerebral malaria in mice. Nat Commun 13:4028 (2022). PubMed: 35821013
- Ma C et al. Heme oxygenase-1 modulates ferroptosis by fine-tuning levels of intracellular iron and reactive oxygen species of macrophages in response to Bacillus Calmette-Guerin infection. Front Cell Infect Microbiol 12:1004148 (2022). PubMed: 36211962
- Canesin G et al. Measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractions. STAR Protoc 2:100491 (2021). PubMed: 33997811
- Kaur P et al. Activated heme synthesis regulates glycolysis and oxidative metabolism in breast and ovarian cancer cells. PLoS One 16:e0260400 (2021). PubMed: 34807950
- Pettway YD et al. Early life stress induces dysregulation of the heme pathway in adult mice. Physiol Rep 9:e14844 (2021). PubMed: 34042301