Anti-Heme oxygenase 2抗体(ab90515)

概述

  • 产品名称Anti-Heme oxygenase 2抗体
    参阅全部 Heme oxygenase 2 一抗
  • 描述
    兔多克隆抗体to Heme oxygenase 2
  • 经测试应用适用于: WB, IP, ELISA, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Hamster, Dog, Human, Pig, Monkey
  • 免疫原

    Synthetic peptide derived from a sequence near the amino-terminus of Human Heme oxygenase 2.

  • 阳性对照
    • MCF7 cells, human breast cancer tissue, Human, mouse, Rat and Dog Liver Microsome Extracts.

性能

应用

Our Abpromise guarantee covers the use of ab90515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 36 kDa.
IP Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.
  • 序列相似性Belongs to the heme oxygenase family.
    Contains 2 HRM (heme regulatory motif) repeats.
  • 细胞定位Microsome. Endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Heme oxygenase (decycling) 2 antibody
    • Heme oxygenase (decyclizing) 2 antibody
    • Heme oxygenase 2 antibody
    • HMOX 2 antibody
    • Hmox2 antibody
    • HMOX2 protein antibody
    • HMOX2_HUMAN antibody
    • HO 2 antibody
    • HO-2 antibody
    • HO2 antibody
    • OTTHUMP00000159847 antibody
    see all

Anti-Heme oxygenase 2 antibody 图像

  • ab90515 staining Heme oxygenase 2 in MCF7 cells by Immunofluorescence (green). Nuclei are stained in blue pseudocolor using DRAQ5.
  • All lanes : Anti-Heme oxygenase 2 antibody (ab90515) at 1/1000 dilution

    Lane 1 : Heme oxygenase 2 Recombinant Human Protein
    Lane 2 : Human Liver Microsome Extract
    Lane 3 : Mouse Liver Microsome Extract
    Lane 4 : Rat Liver Microsome Extract
    Lane 5 : Dog Liver Microsome Extract


    Predicted band size : 36 kDa

Anti-Heme oxygenase 2 antibody (ab90515)参考文献

ab90515 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (Hep G2, liver cancer)
Gel Running Conditions Reduced Denaturing
Loading amount 25 µg
Specification Hep G2, liver cancer
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 19 2016

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Hep G2)
Specification Hep G2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Dec 29 2014

Following up my message from a week ago regarding ab90515, Anti-Heme oxygenase 2 antibody, the immunogen, though we were not able to obtain the exact sequence, is within the first 50 amino acids of Human HO-2.

I hope this helps. If you have ...

Read More

Thank you for your interest in this HO-2 antibody. I am waiting for our source to send the immunogen information, which I will forward.

We do not have a non-rabbit/mouse hosted antibody that has been tested for IHC but this otherwise appears...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (lung supernatant)
Loading amount 85 µg
Specification lung supernatant
Treatment hyperoxia for 18 hrs
Gel Running Conditions Reduced Denaturing (12,5%)
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Sandra Sobocanec

Verified customer

提交于 May 17 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"