Anti-Heme oxygenase 2抗体(ab86131)

概述

  • 产品名称Anti-Heme oxygenase 2抗体
    参阅全部 Heme oxygenase 2 一抗
  • 描述
    兔多克隆抗体to Heme oxygenase 2
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Heme oxygenase 2.

    (Peptide available as ab95843.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; HEK293; Jurkat.

性能

应用

Our Abpromise guarantee covers the use of ab86131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa).
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.
  • 序列相似性Belongs to the heme oxygenase family.
    Contains 2 HRM (heme regulatory motif) repeats.
  • 细胞定位Microsome. Endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Heme oxygenase (decycling) 2 antibody
    • Heme oxygenase (decyclizing) 2 antibody
    • Heme oxygenase 2 antibody
    • HMOX 2 antibody
    • Hmox2 antibody
    • HMOX2 protein antibody
    • HMOX2_HUMAN antibody
    • HO 2 antibody
    • HO-2 antibody
    • HO2 antibody
    • OTTHUMP00000159847 antibody
    see all

Anti-Heme oxygenase 2 antibody 图像

  • All lanes : Anti-Heme oxygenase 2 antibody (ab86131) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 16 kDa,23 kDa,31 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab86131 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86131, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HepG2 cells at 5µg/ml.

Anti-Heme oxygenase 2 antibody (ab86131)参考文献

ab86131 has not yet been referenced specifically in any publications.

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