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Our Abpromise guarantee covers the use of ab12220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 5 - 10 µg/ml. Predicted molecular weight: 32.8 kDa.|
|IHC-P||Use a concentration of 5 - 10 µg/ml.|
|IHC-Fr||Use a concentration of 5 - 10 µg/ml.|
|Inhibition Assay||Use at an assay dependent concentration. This antibody will inhibit the activity of human or rat heme oxygenase 1.|