RabMAb

Anti-Heme Oxygenase 1抗体[EPR1390Y] (ab68477)

概述

  • 产品名称Anti-Heme Oxygenase 1抗体[EPR1390Y]
    参阅全部 Heme Oxygenase 1 一抗
  • 描述
    兔单克隆抗体[EPR1390Y] to Heme Oxygenase 1
  • 经测试应用适用于: ICC/IF, WB, IPmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    within Human Heme Oxygenase 1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P09601

  • 阳性对照
    • HepG2, A549, rat kidney, rat spleen, mouse kidney cell lysate.
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab68477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
WB 1/10000 - 1/50000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IP 1/20.
  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.
    • 序列相似性Belongs to the heme oxygenase family.
    • 细胞定位Microsome. Endoplasmic reticulum.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 32 kD antibody
      • bK286B10 antibody
      • D8Wsu38e antibody
      • heat shock protein 32 kD antibody
      • heat shock protein 32kD antibody
      • Heat shock protein antibody
      • Heme oxygenase (decycling) 1 antibody
      • Heme oxygenase 1 antibody
      • Hemox antibody
      • HMOX 1 antibody
      • Hmox antibody
      • Hmox1 antibody
      • HMOX1_HUMAN antibody
      • HO 1 antibody
      • HO antibody
      • HO-1 antibody
      • HO1 antibody
      • Hsp32 antibody
      see all

    Anti-Heme Oxygenase 1 antibody [EPR1390Y] 图像

    • ab68477 (unpurified) staining Heme Oxygenase 1 in mouse embryonic fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1.5% serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/100 in 1X HBSS + 0.02% Triton X-100 + 1.5% FBS) for 3 hours at 25°C. An Alexa Fluor® 594-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • Immunocytochemistry/Immunofluorescence analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Heme Oxygenase 1 with purified ab68477 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

      Negative control 1: PBS only.

    • ab68477 (purified) at 1/20 immunoprecipitating Heme Oxygenase 1 in A549 (Human lung carcinoma cell line) whole cell lysate.

      Lane 1 (input): A549 whole cell lysate (10ug).

      Lane 2 (+): ab68477 + A549 whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133267 in HeLa whole cell lysate.

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/20000 dilution (purified)

      Lane 1 : Rat kidney lysate
      Lane 2 : Rat spleen lysate
      Lane 3 : Mouse kidney lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size : 33 kDa
      Observed band size : 33 kDa

      Blocking and dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/20000 dilution (purified)

      Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size : 33 kDa
      Observed band size : 33 kDa

      Blocking and dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/2000 dilution (purified)

      Lane 1 : HepG2 cell lysate
      Lane 2 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP conjugated Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 33 kDa
      Observed band size : 33 kDa
      Additional bands at : 125-130 kDa,30 kDa,48 kDa,52 kDa. We are unsure as to the identity of these extra bands.

    Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)参考文献

    This product has been referenced in:
    • He C  et al. Vasoprotective effect of PDGF-CC mediated by HMOX1 rescues retinal degeneration. Proc Natl Acad Sci U S A 111:14806-11 (2014). WB . Read more (PubMed: 25267616) »
    • Maruyama A  et al. Non-coding RNA derived from the region adjacent to the human HO-1 E2 enhancer selectively regulates HO-1 gene induction by modulating Pol II binding. Nucleic Acids Res 42:13599-614 (2014). Read more (PubMed: 25404134) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Sample Human Tissue lysate - whole (brain)
    Gel Running Conditions Reduced Denaturing (12)
    Loading amount 50 µg
    Specification brain
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    提交于 May 13 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (rat intestinal epithelial cells (RIE-1))
    Permeabilization Yes - 0.2% TritonX-100
    Specification rat intestinal epithelial cells (RIE-1)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
    Fixative Paraformaldehyde
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    提交于 Feb 12 2016

    Application Immunocytochemistry/ Immunofluorescence
    Sample Chinese Hamster Cell (Chinese hamster ovary cell)
    Permeabilization Yes - 0.2% TritonX-100
    Specification Chinese hamster ovary cell
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
    Fixative Paraformaldehyde
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    提交于 Feb 12 2016

    Application Western blot
    Sample Spodoptera frugiperda (SF9 cells) Cell lysate - whole cell (sf9 cells)
    Gel Running Conditions Reduced Denaturing
    Loading amount 100000 cells
    Treatment 30 nM Bortezomib for 16 hours
    Specification sf9 cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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    提交于 Feb 09 2016

    Application Western blot
    Sample Rat Cell lysate - whole cell (rat intestinal epithelial cells (RIE-1))
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Loading amount 100000 cells
    Treatment 100 nM Bortezomib for 16 hours
    Specification rat intestinal epithelial cells (RIE-1)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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    Verified customer

    提交于 Feb 09 2016

    Application Western blot
    Sample Chinese Hamster Cell lysate - whole cell (Chinese hamster ovary cell)
    Gel Running Conditions Reduced Denaturing
    Loading amount 100000 cells
    Treatment 100 nM Bortezomib for 16 hours
    Specification Chinese hamster ovary cell
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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    提交于 Feb 09 2016

    Application Flow Cytometry
    Sample Mouse Cell (Mouse Lewis Lung Carcinoma cells)
    Permeabilization Yes - 0.2% Triton X-100
    Gating Strategy No gating
    Specification Mouse Lewis Lung Carcinoma cells
    Preparation Cell harvesting/tissue preparation method: trypsin
    Sample buffer: Hanks’ Balanced Salt solution (HBSS)
    Fixation Paraformaldehyde
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    提交于 Jan 22 2016

    Application IHC - Wholemount
    Sample Mouse Tissue (Transplanted lung tumor section)
    Specification Transplanted lung tumor section
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    提交于 Dec 21 2015

    Application IHC - Wholemount
    Sample Human Tissue (Breast invasive ductal carcinoma)
    Specification Breast invasive ductal carcinoma
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    Verified customer

    提交于 Dec 21 2015

    Application Western blot
    Sample African Green Monkey Cell lysate - whole cell (COS-7 cells)
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Loading amount 100000 cells
    Treatment 30 nM Bortezomib for 24 hours
    Specification COS-7 cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
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    提交于 Jun 22 2015

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"