The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.
Immunocytochemistry/Immunofluorescence analysis of heat shocked HeLa cells using ab79854 at 1/100 dilution for 12 hours at 4°C. Fixation: 2% Formaldehyde for 20 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytoplasm. Magnification: 100x. Heat Shocked at 42°C for 1h.
Wang X et al. Activation of the Nuclear Factor E2-Related Factor 2/Antioxidant Response Element Pathway is Neuroprotective Following Spinal Cord Injury. J Neurotrauma : (2011).
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