概述

  • 产品名称
    Anti-Heme Oxygenase 1抗体
    参阅全部 Heme Oxygenase 1 一抗
  • 描述
    兔多克隆抗体to Heme Oxygenase 1
  • 经测试应用
    适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human
  • 免疫原

    Multi-antigenic synthetic peptide from Human Heme Oxygenase 1

  • 阳性对照
    • Lysate from heat shocked HeLa cells

应用

Our Abpromise guarantee covers the use of ab79854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
IHC-P 1/50.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.

靶标

  • 功能
    Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.
  • 序列相似性
    Belongs to the heme oxygenase family.
  • 细胞定位
    Microsome. Endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 32 kD antibody
    • bK286B10 antibody
    • D8Wsu38e antibody
    • heat shock protein 32 kD antibody
    • heat shock protein 32kD antibody
    • Heat shock protein antibody
    • Heme oxygenase (decycling) 1 antibody
    • Heme oxygenase 1 antibody
    • Hemox antibody
    • HMOX 1 antibody
    • Hmox antibody
    • Hmox1 antibody
    • HMOX1_HUMAN antibody
    • HO 1 antibody
    • HO antibody
    • HO-1 antibody
    • HO1 antibody
    • Hsp32 antibody
    see all

Anti-Heme Oxygenase 1 antibody 图像

  • All lanes : Anti-Heme Oxygenase 1 antibody (ab79854) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Cell lysates prepared from human A431, A549, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3 and T98G cells


    Predicted band size : 33 kDa
  • Immunocytochemistry/Immunofluorescence analysis of heat shocked HeLa cells using ab79854 at 1/100 dilution for 12 hours at 4°C. Fixation: 2% Formaldehyde for 20 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytoplasm. Magnification: 100x. Heat Shocked at 42°C for 1h.

Anti-Heme Oxygenase 1 antibody (ab79854)参考文献

This product has been referenced in:
  • Ni W  et al. Role of Erythrocyte CD47 in Intracerebral Hematoma Clearance. Stroke 47:505-11 (2016). WB, IHC-FoFr ; Mouse . Read more (PubMed: 26732568) »
  • Wang X  et al. Activation of the Nuclear Factor E2-Related Factor 2/Antioxidant Response Element Pathway is Neuroprotective Following Spinal Cord Injury. J Neurotrauma : (2011). WB ; Rat . Read more (PubMed: 21806470) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (brain microsomes spleen cytosol)
Loading amount
75 µg
Specification
brain microsomes spleen cytosol
Treatment
hyperoxia (>95%O2) for 18hrs
Gel Running Conditions
Reduced Denaturing (gel 12,5%)
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Sandra Sobocanec

Verified customer

提交于 Oct 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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