概述

  • 产品名称
    Anti-Hemagglutinin抗体
    参阅全部 Hemagglutinin 一抗
  • 描述
    兔多克隆抗体to Hemagglutinin
  • 经测试应用
    适用于: ELISA, ICC/IF, WBmore details
  • 免疫原

    Synthetic peptide:

    YPYDVPDYA

    conjugated to KLH, corresponding to Internal sequence amino acids 99-107 of Hemagglutinin.

  • 常规说明


    The antibody ab20084 is able to recognise the HA epitope when used to tag other proteins.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • Primary antibody说明
    The antibody ab20084 is able to recognise the HA epitope when used to tag other proteins.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab20084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/70000. The anti HA diluted 1/70,000 gave an OD=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification.
ICC/IF Use at an assay dependent dilution.
WB 1/5000. Predicted molecular weight: 1 kDa.

靶标

  • 相关性
    Hemagglutinin (HA) is a class I viral fusion protein from Influenza virus. It is a major glycoprotein, comprising over 80% of the envelope proteins present in the virus particle. HA binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell, and is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. The extent of infection into host organism is determined by HA. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoproteases secreted by the bronchial epithelial cells. The HA protein is a homotrimer of disulfide-linked HA1-HA2. It also plays a major role in the determination of host range restriction and virulence. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains.
  • 细胞定位
    Cell membrane; apical cell membrane; single-pass type I membrane protein.
  • 别名
    • HA antibody
    • HA1 antibody
    • HA2 antibody
    • Hemagglutinin antibody

图片

  • Immunofluorescence analysis of influenza virus-like particle producer cells, staining Hemagglutinin (red) with ab20084.

    Cells were fixed in paraformaldehyde for 10 min and permeabilized in 0.05% Triton-X 100 for 1 min. After blocking with 1% gelatin, the cells were incubated with primary antibody, followed by incubation with Cy3-conjugated goat anti-rabbit IgG as the secondary antibody.

文献

This product has been referenced in:
  • Asensio-Juan E  et al. The histone demethylase PHF8 is a molecular safeguard of the IFN? response. Nucleic Acids Res 45:3800-3811 (2017). Read more (PubMed: 28100697) »
  • Wang R  et al. Protein Inhibitor of Activated STAT3 Suppresses Oxidized LDL-induced Cell Responses during Atherosclerosis in Apolipoprotein E-deficient Mice. Sci Rep 6:36790 (2016). WB . Read more (PubMed: 27845432) »

See all 6 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton
Specification
HeLa
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 01 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
50 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 23 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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