The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/5000.
Use at an assay dependent concentration.
Hemagglutinin (HA) is a class I viral fusion protein from Influenza virus. It is a major glycoprotein, comprising over 80% of the envelope proteins present in the virus particle. HA binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell, and is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. The extent of infection into host organism is determined by HA. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoproteases secreted by the bronchial epithelial cells. The HA protein is a homotrimer of disulfide-linked HA1-HA2. It also plays a major role in the determination of host range restriction and virulence. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains.
Cell membrane; apical cell membrane; single-pass type I membrane protein.
Immunocytochemistry/ Immunofluorescence - Anti-Hemagglutinin antibody [16B12] (DyLight® 488) (ab117488)This image is courtesy of an Abreview submitted by Ya Zhou
ab117488 staining Hemagglutinin in Monkey cos7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde. Samples were incubated with primary antibody (1/100) for 1 hour at 22°C.