Rat, Cow, Dog, Zebrafish
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse HELT.
This antibody gave a positive signal in the following tissue lysates: Mouse brain; Mouse testis; Mouse heart; Human heart.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 27 kDa).
Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGCG-3'.
Belongs to the HEY family.
Contains 1 basic helix-loop-helix (bHLH) domain. Contains 1 Orange domain.
Information by UniProt
Hairy and enhancer of split-related protein HELT antibody
Western blot - Anti-HELT antibody (ab101842)
All lanes :
Anti-HELT antibody (ab101842) at 1 µg/ml
Lane 1 :
Brain (Mouse) Tissue Lysate
Lane 2 :
Testis (Mouse) Tissue Lysate
Lane 3 :
Heart (Mouse) Tissue Lysate
Lane 4 :
Human heart tissue lysate - total protein (
Lysates/proteins at 10 µg per lane.
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
34 kDa (
why is the actual band size different from the predicted?
Exposure time :
8 minutesPlease note the current predicted molecular weight for this protein is 27kDa in Mouse and 36kDa in Human.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"