产品名称 Anti-HELT抗体 参阅全部 HELT 一抗
WB more details
Rat, Cow, Dog, Zebrafish
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse HELT.
This antibody gave a positive signal in the following tissue lysates: Mouse brain; Mouse testis; Mouse heart; Human heart.
存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 27 kDa).
功能 Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGCG-3'.
序列相似性 Belongs to the HEY family. Contains 1 basic helix-loop-helix (bHLH) domain. Contains 1 Orange domain.
Information by UniProt
Hairy and enhancer of split-related protein HELT antibody
Anti-HELT antibody 图像
Western blot - Anti-HELT antibody (ab101842)
All lanes : Anti-HELT antibody (ab101842) at 1 µg/ml Lane 1 : Brain (Mouse) Tissue Lysate Lane 2 : Testis (Mouse) Tissue Lysate Lane 3 : Heart (Mouse) Tissue Lysate Lane 4 : Human heart tissue lysate - total protein ( ab29431) Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 27 kDa Observed band size : 34 kDa ( why is the actual band size different from the predicted?) Exposure time : 8 minutes Please note the current predicted molecular weight for this protein is 27kDa in Mouse and 36kDa in Human.
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