Anti-Helicobacter pylori urease抗体(ab51954)

概述

  • 产品名称Anti-Helicobacter pylori urease抗体
  • 描述
    鸭多克隆抗体to Helicobacter pylori urease
  • 经测试应用适用于: WB, ELISA, Immunodiffusion, Immunoelectrophoresismore details
  • 种属反应性
    Reacts with Helicobacter pylori.
  • 免疫原

    Helicobacter pylori urease

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 10mM PBS, pH 7.0
  • Concentration information loading...
  • 纯度>90% by SDS-PAGE
  • 克隆多克隆
  • 同种型IgY
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab51954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.
ELISA Use a concentration of 1 mg/ml.
Immunodiffusion Use at an assay dependent dilution.
Immunoelectrophoresis Use at an assay dependent dilution.

靶标

  • 相关性Helicobacter pylori is a Gram-negative, microaerophilic bacterium that inhabits various areas of the stomach and duodenum. It causes a chronic low-level inflammation of the stomach lining and is strongly linked to the development of duodenal and gastric ulcers and stomach cancer. Over 80% of individuals infected with the bacterium are asymptomatic. Urease is a heterohexamer of 3 ureA (alpha) and 3 ureB (beta) subunits. Four heterohexamers assemble to form a 16 nm dodecameric complex. It is a nickel-dependent metalloenzyme that catalyzes the hydrolysis of urea to form ammonia and carbon dioxide. The ammonia produced by ureolysis increases the gastric pH thereby providing an environment permissive for colonization of the stomach. The dodecameric complex is active at pH 3.0. The novel dodecameric structure of the enzyme may allow it to remain active at the cell surface at acidic gastric pH. Within this dodecameric structure the 12 active sites are clustered within the interior of the proteinaceous shell. This may allow a high local concentration of ammonia within the enzyme which may protect the nickel-chelating groups from protonation.
  • 细胞定位Cytoplasmic: associates with the outer membrane upon autolysis of neighboring bacteria.
  • 别名
    • Bifunctional urease subunit gamma/beta antibody
    • Urea amidohydrolase subunit alpha antibody
    • ureA antibody
    • Urease subunit alpha antibody
    • ureC antibody
    see all

Anti-Helicobacter pylori urease antibody (ab51954)参考文献

This product has been referenced in:
  • Lee HK  et al. Growth inhibitory, bactericidal, and morphostructural effects of dehydrocostus lactone from Magnolia sieboldii Leaves on antibiotic-susceptible and -resistant strains of Helicobacter pylori. PLoS One 9:e95530 (2014). WB . Read more (PubMed: 24747984) »
  • Ngan LT  et al. Growth-inhibiting, bactericidal, and urease inhibitory effects of Paeonia lactiflora root constituents and related compounds on antibiotic-susceptible and -resistant strains of Helicobacter pylori. J Agric Food Chem 60:9062-73 (2012). WB . Read more (PubMed: 22891951) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"