HeLa核extract裂解液(TPA stimulated 2 hr) (ab14653)

概述

  • 产品名称HeLa核extract裂解液(TPA stimulated 2 hr)
    参阅全部 HeLa 细胞裂解液
  • 描述
    HeLa核extract裂解液
  • 常规说明Cells were cultured in medium supplemented with 50 ng/mL TPA (phorbol, 12 myristate, 13 acetate) for two hours at 37°C immediately prior to harvesting. We recommend aliquoting the extracts into single use fractions and then storing.
  • 经测试应用适用于: WB, EMSAmore details

性能

  • 形式Liquid
  • 存放说明Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • 研究领域
  • 背景HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.

应用

Our Abpromise guarantee covers the use of ab14653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.
EMSA Use at an assay dependent dilution.

HeLa nuclear extract lysate (TPA stimulated 2 hr) (ab14653)参考文献

ab14653 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"