HeLa核extract裂解物(4 hr serum response) (ab14651)
概述
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产品名称
HeLa核extract裂解物(4 hr serum response)
参阅全部 HeLa 细胞裂解液 -
常规说明
Cells were cultured for 24 hours in low serum (0.5%) conditions and then serum stimulated (10%) for 4 hours prior to harvesting. We recommend aliquoting the extracts into single use fractions and then storing them.
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.90
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol -
Concentration information loading...
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研究领域
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背景
HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab14651 尚未被引用在任何文献中。