We recommend aliquoting the extracts into single-use fractions and then storing them.
Cells were cultured for 24 hours in low serum (0.5%) conditions and then serum stimulated (10%) for 2 hours immediately prior to harvesting.
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17.
HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.
HeLa nuclear extract lysate (2 hr serum response) (ab14650)参考文献
has not yet been referenced specifically in any publications.