HeLa核extract裂解液(2 hr serum response) (ab14650)

概述

  • 产品名称HeLa核extract裂解液(2 hr serum response)
    参阅全部 HeLa 细胞裂解液
  • 描述
    HeLa核extract裂解液
  • 常规说明We recommend aliquoting the extracts into single-use fractions and then storing them. Cells were cultured for 24 hours in low serum (0.5%) conditions and then serum stimulated (10%) for 2 hours immediately prior to harvesting.

性能

  • 形式Liquid
  • 存放说明Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • 研究领域
  • 背景HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.

HeLa nuclear extract lysate (2 hr serum response) (ab14650)参考文献

ab14650 has not yet been referenced specifically in any publications.

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