HEK293 (人)全细胞裂解液- expressing HA tag protein (ab20896)

概述

性能

  • 背景Human Embryonic Kidney (HEK) 293 cells were originally derived from human embryonic kidney and subsequently demonstrated to be a useful cell type to produce adenovirus, other viral vectors, and effectively glycosylated human recombinant proteins. They are therefore used for virology studies and transfected to express recombinant proteins for studies in a number of research fields.

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应用

Our Abpromise guarantee covers the use of ab20896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. ab20896 is a lysate produced from 293 cells transfected with a 55 kDa HA-tagged minigene. Pretreatment 10 min in 95 degree cersius is recommended before loading on SDS-PAGE for Western blotting. 10 µg per lane is recommended for mini gels.

HEK293 (Human) Whole Cell Lysate - expressing HA tag protein 图像

  • All lanes : Anti-HA tag antibody (ab13834) at 1/500 dilution

    Lane 1 : HEK293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896)
    Lane 2 : HEK293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896) with HA tag peptide (ab13835) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Alexa Fluor 680 Goat-anti-Rabbit IgG at 1/5000 dilution

    Performed under reducing conditions.

    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

HEK293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896)参考文献

ab20896 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.
In this case only the protein concentration was determined and not the HA protein specifically. Unofortunately we do not have any pure HA proteins on our catalog to use in your studies.


I hope this informa...

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Thank you for your patience.

We haveone protein in our catalog currently which has a HA tag and could be useful for you. It is the ab116535, a tagged CARHSP1 protein which runs at 44kD in Western blot. http://www.abcam.com/index.html?datashee...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"