Anti-HECW1抗体(ab121265)

概述

  • 产品名称Anti-HECW1抗体
    参阅全部 HECW1 一抗
  • 描述
    兔多克隆抗体to HECW1
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence, corresponding to amino acids 356-481 of Human HECW1.

  • 阳性对照
    • Human pancreas tissue; A431 cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab121265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

靶标

  • 功能E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent degradation of DVL1. Also targets the mutant SOD1 protein involved in familial amyotrophic lateral sclerosis (FALS). Forms cytotoxic aggregates with DVL1, SSR3 and mutant SOD1 that lead to motor neuron death in FALS.
  • 组织特异性Predominantly expressed in neurons of adult and fetal brain. Weakly expressed in the kidney.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 2 WW domains.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C2 and WW domain-containing protein 1 antibody
    • E3 ubiquitin-protein ligase HECW1 antibody
    • HECT antibody
    • HECT type E3 ubiquitin ligase antibody
    • HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 antibody
    • HECT, C2 and WW domain-containing protein 1 antibody
    • HECW 1 antibody
    • Hecw1 antibody
    • HECW1_HUMAN antibody
    • hNEDL1 antibody
    • NEDD4 like ubiquitin protein ligase 1 antibody
    • NEDD4-like E3 ubiquitin-protein ligase 1 antibody
    • NEDL1 antibody
    see all

Anti-HECW1 antibody 图像

  • ab121265 at 1/25 dilution staining HECW1 in Paraffin-embedded Human pancreas tissue by Immunohistochemistry.
  • ab121265 at 4 µg/ml staining HECW1 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).

Anti-HECW1 antibody (ab121265)参考文献

ab121265 has not yet been referenced specifically in any publications.

Product Wall

Thank very much for your email. First, I would like to apologize as I have no idea why I pasted HAT1 sequence in my email; a totally unrelated antibody because we also take phone calls so maybe there was mix up. I apologize for any inconvenience.
...

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Thank you for contacting us.
The full sequence of Human HAT1 as follows:
>sp|O14929|HAT1_HUMAN Histone acetyltransferase type B catalytic subunit OS=Homo sapiens GN=HAT1 PE=1 SV=1
MAGFGAMEKFLVEYKSAVEKKLAEYKCNTNTAIELKLVRFPEDLENDIRTFFPEYTH...

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Thank you for contacting us.
The main difference between the two is Immunogen. The immunogen of ab12164 corresponds to amino acid 500-614 and for ab121265 is from aa 356-481.
The other difference is conc. of antibodies.
I am glad to hear...

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