概述

  • 产品名称Anti-HECTD2抗体
    参阅全部 HECTD2 一抗
  • 描述
    兔多克隆抗体to HECTD2
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 684 - 733 (TDLTIKYFWD VVLGFPLDLQ KKLLHFTTGS DRVPVGGMAD LNFKISKNET) of human HECTD2 (NP_877497).

  • 阳性对照
    • Fetal muscle lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab82060 is purified by a peptide affinity chromatography method.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 88 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

靶标

Anti-HECTD2 antibody 图像

  • Anti-HECTD2 antibody (ab82060) at 1 µg/ml + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 88 kDa
    Observed band size : 88 kDa

Anti-HECTD2 antibody (ab82060)参考文献

This product has been referenced in:
  • Sun T  et al. MiR-221 promotes the development of androgen independence in prostate cancer cells via downregulation of HECTD2 and RAB1A. Oncogene N/A:N/A (2013). Read more (PubMed: 23770851) »

See 1 Publication for this product

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