Anti-HEC1/HEC (phospho S165)抗体(ab233459)
Key features and details
- Rabbit polyclonal to HEC1/HEC (phospho S165)
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-HEC1/HEC (phospho S165)抗体
参阅全部 HEC1/HEC 一抗 -
描述
兔多克隆抗体to HEC1/HEC (phospho S165) -
宿主
Rabbit -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human HEC1/HEC (phospho S165) conjugated to keyhole limpet haemocyanin.
Database link: O14777 -
阳性对照
- ICC/IF: MCF10A cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab233459于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/150.
|
说明 |
---|
ICC/IF
1/150. |
靶标
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功能
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. -
序列相似性
Belongs to the NDC80/HEC1 family. -
发展阶段
Expression peaks in mitosis. -
翻译后修饰
Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB. -
细胞定位
Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. - Information by UniProt
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数据库链接
- Entrez Gene: 10403 Human
- Entrez Gene: 67052 Mouse
- Entrez Gene: 301701 Rat
- Omim: 607272 Human
- SwissProt: O14777 Human
- SwissProt: Q9D0F1 Mouse
- Unigene: 414407 Human
- Unigene: 225956 Mouse
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别名
- from Entrez Gene ; antibody
- HEC antibody
- Highly expressed in cancer antibody
see all
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab233459 尚未被引用在任何文献中。