RabMAb

Anti-HEC1抗体[EPR5342] (ab109496)

概述

  • 产品名称Anti-HEC1抗体[EPR5342]
    参阅全部 HEC1 一抗
  • 描述
    兔单克隆抗体[EPR5342] to HEC1
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C-terminus of Human HEC1.

  • 阳性对照
    • Jurkat, HeLa, NCCIT, and Human spleen lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab109496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 74 kDa.
IP 1/10 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IHC-P.
  • 靶标

    • 功能Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
    • 序列相似性Belongs to the NDC80/HEC1 family.
    • 发展阶段Expression peaks in mitosis.
    • 翻译后修饰Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.
    • 细胞定位Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
    • Information by UniProt
    • 数据库链接
    • 别名
      • from Entrez Gene ; antibody
      • HEC antibody
      • Highly expressed in cancer antibody
      • Highly expressed in cancer protein antibody
      • Highly expressed in cancer rich in leucine heptad repeats antibody
      • HsHec1 antibody
      • hsNDC80 antibody
      • Kinetochore associated 2 antibody
      • Kinetochore associated protein 2 antibody
      • Kinetochore protein Hec1 antibody
      • Kinetochore protein NDC80 homolog antibody
      • Kinetochore-associated protein 2 antibody
      • KNTC2 antibody
      • ndc80 antibody
      • NDC80 homolog kinetochore complex component antibody
      • NDC80 kinetochore complex component homolog antibody
      • NDC80, S. cerevisiae, homolog of antibody
      • NDC80_HUMAN antibody
      • Retinoblastoma associated protein HEC antibody
      • Retinoblastoma-associated protein HEC antibody
      • TID3 antibody
      see all

    Anti-HEC1 antibody [EPR5342] 图像

    • All lanes : Anti-HEC1 antibody [EPR5342] (ab109496) at 1/10000 dilution

      Lane 1 : Jurkat lysate
      Lane 2 : HeLa lysate
      Lane 3 : NCCIT lysate
      Lane 4 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 74 kDa

    Anti-HEC1 antibody [EPR5342] (ab109496)参考文献

    ab109496 has not yet been referenced specifically in any publications.

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