概述
- 产品名称Anti-HEC1抗体
参阅全部 HEC1 一抗 - 描述山羊多克隆抗体to HEC1
- 经测试应用适用于: WB, IPmore details
- 种属反应性与反应: Mouse, Human
预测可用于: Chimpanzee, Gorilla, Orangutan
- 免疫原
Synthetic peptide, corresponding to a region within C terminal amino acids 600-642 of Human HEC1 (NP_006092.1)
- 阳性对照
- HeLa, 293T or NIH3T3 cell lysate
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- 存储溶液Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... - 纯度Immunogen affinity purified
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
- Positive Controls
应用
Our Abpromise guarantee covers the use of ab97610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 74 kDa. | |
| IP | Use a concentration of 2 - 5 µg/ml. |
靶标
- 功能Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
- 序列相似性Belongs to the NDC80/HEC1 family.
- 发展阶段Expression peaks in mitosis.
- 翻译后修饰Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.
- 细胞定位Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
- Information by UniProt
-
数据库链接
- Entrez Gene: 10403 Human
- Entrez Gene: 67052 Mouse
- Omim: 607272 Human
- SwissProt: O14777 Human
- SwissProt: Q9D0F1 Mouse
- Unigene: 414407 Human
- Unigene: 225956 Mouse
-
别名
- from Entrez Gene ; antibody
- HEC antibody
- Highly expressed in cancer antibody
see all
Anti-HEC1 antibody 图像
-
Western blot - HEC1 antibody (ab97610)All lanes : Anti-HEC1 antibody (ab97610) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size : 74 kDa -
Immunoprecipitation - HEC1 antibody (ab97610)Lane 1: 3µg ab97610 were used to precipitate 1mg HeLa whole cell lysate. 20% IP was loaded and probed with ab97610 at 1µg/ml.
Lane 2: same, using a control IgG.
Detection: chemiluminescence with exposure time of 10 seconds.
Anti-HEC1 antibody (ab97610)参考文献
ab97610 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"