概述

  • 产品名称
    Anti-HEC1抗体
    参阅全部 HEC1 一抗
  • 描述
    山羊多克隆抗体to HEC1
  • 宿主
    Goat
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within C terminal amino acids 600-642 of Human HEC1 (NP_006092.1)

  • 阳性对照
    • HeLa, 293T or NIH3T3 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab97610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 74 kDa.
IP Use a concentration of 2 - 5 µg/ml.

靶标

  • 功能
    Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
  • 序列相似性
    Belongs to the NDC80/HEC1 family.
  • 发展阶段
    Expression peaks in mitosis.
  • 翻译后修饰
    Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.
  • 细胞定位
    Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
  • Information by UniProt
  • 数据库链接
    • 别名
      • from Entrez Gene ; antibody
      • HEC antibody
      • Highly expressed in cancer antibody
      • Highly expressed in cancer protein antibody
      • Highly expressed in cancer rich in leucine heptad repeats antibody
      • HsHec1 antibody
      • hsNDC80 antibody
      • Kinetochore associated 2 antibody
      • Kinetochore associated protein 2 antibody
      • Kinetochore protein Hec1 antibody
      • Kinetochore protein NDC80 homolog antibody
      • Kinetochore-associated protein 2 antibody
      • KNTC2 antibody
      • ndc80 antibody
      • NDC80 homolog kinetochore complex component antibody
      • NDC80 kinetochore complex component homolog antibody
      • NDC80, S. cerevisiae, homolog of antibody
      • NDC80_HUMAN antibody
      • Retinoblastoma associated protein HEC antibody
      • Retinoblastoma-associated protein HEC antibody
      • TID3 antibody
      see all

    图片

    • Western blot - HEC1 antibody (ab97610)
      Western blot - HEC1 antibody (ab97610)
      All lanes : Anti-HEC1 antibody (ab97610) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg

      Predicted band size: 74 kDa

    • Immunoprecipitation - HEC1 antibody (ab97610)
      Immunoprecipitation - HEC1 antibody (ab97610)
      Lane 1: 3µg ab97610 were used to precipitate 1mg HeLa whole cell lysate. 20% IP was loaded and probed with ab97610 at 1µg/ml.
      Lane 2: same, using a control IgG.
      Detection: chemiluminescence with exposure time of 10 seconds.

    文献

    ab97610 has not yet been referenced specifically in any publications.

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