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Our Abpromise guarantee covers the use of ab3613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|IP||Use at an assay dependent concentration.|
|WB||1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 74 kDa).|
|ICC/IF||Use at an assay dependent concentration.|
ab3613 at 1/1000 dilution staining HeLa cells by ICC/IF. The cells were formaldehyde fixed and blocked with 5% BSA prior to incubation with the antibody for 2 hours. An Alexa-Fluor ® 488 conjugated goat anti-mouse antibody was used as the secondary.