Anti-heavy chain cardiac Myosin抗体[BA-G5] (ab50967)

概述

  • 产品名称Anti-heavy chain cardiac Myosin抗体[BA-G5]
    参阅全部 heavy chain cardiac Myosin 一抗
  • 描述
    小鼠单克隆抗体[BA-G5] to heavy chain cardiac Myosin
  • 经测试应用适用于: WB, IHC-P, IHC-FoFr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human
    预测可用于: Cow
  • 免疫原

    Cow skeletal muscle myosin

  • 阳性对照
    • This antibody gave a positive signal in the following Tissue Lysates: Mouse Skeletal Muscle, Rat Heart, Rat Skeletal Muscle

性能

应用

Our Abpromise guarantee covers the use of ab50967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).
IHC-P 1/100.
IHC-FoFr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 20139090

靶标

Anti-heavy chain cardiac Myosin antibody [BA-G5] 图像

  • Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967) at 5 µg/ml + Heart (Mouse) Tissue Lysate at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Mouse IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 223 kDa
    Observed band size : 223 kDa
  • ab50967 staining heavy chain cardiac Myosin in murine HL-1 cells (derived from an atrial tumor) by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100, blocked with 10% serum for 30 minutes at 21°C and then incubated with ab50967 at a 1/200 dilution for 16 hours at 4°C. The secondary used was a goat anti-mouse polyclonal conjugated to Alexa-Fluor 488, used at a 1/400 dilution. Counterstained with DAPI.

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  • ab50967 staining mouse heart tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer pH 6, prior to blocking with sequential peroxidase and protein block (prediluted) for 20 minutes at 20°C.  The primary antibody was diluted 1/100 and incubated with the sample for 45 minutes at 20°C.  A HRP conjugated goat anti-mouse antibody was used as the secondary.

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  • ab50967 at a 1/200 dilution staining heavy chain cardiac Myosin in murine heterozygote cardiomyocytes by Immunocytochemistry/ Immunofluorescence.
  • All lanes : Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967) at 1 µg/ml

    Lane 1 : Heart (Rat) Tissue Lysate
    Lane 2 : Skeletal Muscle (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 223 kDa
    Observed band size : 223 kDa
    Additional bands at : 48 kDa,95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ab50967 staining heavy chain cardiac Myosin in Mouse whole embryo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 20% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in 5% Goat serum) for 16 hours at 4°C. An undiluted Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.

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Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)参考文献

This product has been referenced in:
  • Nichols CE  et al. Cardiac and mitochondrial dysfunction following acute pulmonary exposure to mountaintop removal mining particulate matter. Am J Physiol Heart Circ Physiol 309:H2017-30 (2015). Read more (PubMed: 26497962) »
  • Ma L  et al. Trap Effect of Three-Dimensional Fibers Network for High Efficient Cancer-Cell Capture. Adv Healthc Mater 4:838-43 (2015). Read more (PubMed: 25645204) »

See all 25 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions Reduced Denaturing
Loading amount 25 µg
Specification Cardiomyocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Sep 27 2016

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Sample Rat Tissue sections (heart)
Specification heart
Permeabilization Yes - Triton x-100, 0.01%
Fixative Formaldehyde
Username

Dr. Shyam Sundar Nandi

Verified customer

提交于 Nov 20 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (mouse whole embryo)
Specification mouse whole embryo
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

提交于 Feb 22 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (mouse heart)
Loading amount 50 µg
Specification mouse heart
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

提交于 Aug 29 2012

Vielen Dank für Ihre Antwortund dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

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Thank you for contacting us. Unfortunately, I would not recommend using ab66470 as a positive control in Western blot: It is a synthetic peptide derived from within residues 600 - 700 of Human beta-cardiac myosin heavy chain. This peptide was used a...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (HL-1 cells (derived from murine atrial tumor))
Specification HL-1 cells (derived from murine atrial tumor)
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X-100 in PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Sep 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (heart smooth muscle)
Loading amount 30 µg
Specification heart smooth muscle
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 16 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (cardiac)
Specification cardiac
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Mar 05 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (heart atria)
Loading amount 15 µg
Specification heart atria
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Prof. Paul Townsend

Verified customer

提交于 Apr 21 2008

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