antigen, corresponding to amino acids 1052-1175 of Human HEATR6.
RT-4 and U-251 MG cell lysates; Human kidney tissue
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/250 - 1/500.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Amplified in breast cancer cell lines MCF7 and BT474.
Contains 4 HEAT repeats.
Information by UniProt
Amplified in breast cancer protein 1 antibody
HEAT repeat-containing protein 6 antibody
Immunocytochemistry/ Immunofluorescence - Anti-HEATR6 antibody (ab122131)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli, nuclear membrane, cytoplasm and golgi apparatus. Recommended concentration of ab122131 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-HEATR6 antibody (ab122131)
All lanes : Anti-HEATR6 antibody (ab122131) at 1/250 dilution Lane 1 : RT-4 cell lysate Lane 2 : U-251 MG cell lysate Lane 3 : Human Plasma Lane 4 : Human Liver lysate Lane 5 : Human Tonsil lysate Developed using the ECL technique
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEATR6 antibody (ab122131)
ab122131, at a 1/200 dilution, staining HEATR6 in paraffin embedded Human kidney tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"