概述

  • 产品名称
    Anti-HEATR6抗体
  • 描述
    兔多克隆抗体to HEATR6
  • 经测试应用
    适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen, corresponding to amino acids 1052-1175 of Human HEATR6.

  • 阳性对照
    • RT-4 and U-251 MG cell lysates; Human kidney tissue

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

图片

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli, nuclear membrane, cytoplasm and golgi apparatus. Recommended concentration of ab122131 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-HEATR6 antibody (ab122131) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique
  • ab122131, at a 1/200 dilution, staining HEATR6 in paraffin embedded Human kidney tissue by Immunohistochemistry.

文献

ab122131 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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