antigen sequence: CFLLEVTTKD PSLVVAGEAL DALFDVFADG KEAERASIQI KLLSALKEFQ PVFKMKIRKE GRGNYSTDQL CVLDNVKMNL RRFIAYQETV EKRL, corresponding to amino acids 585-678 of Human HEATR3.
- Human bone marrow tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Contains 2 HEAT repeats.
Information by UniProt
- HEAT repeat containing 3 antibody
- HEAT repeat-containing protein 3 antibody
- HEAT3_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti-HEATR3 antibody (ab122762)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus, nucleoli and cytoplasm. Recommended concentration of ab122762 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEATR3 antibody (ab122762)
ab122762, at 1/1000 dilution, staining HEATR3 in paraffin-embedded Human bone marrow tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"