antigen, corresponding to amino acids 377-509 of Human HEATR2.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
Contains 10 HEAT repeats.
Information by UniProt
- BC053401 antibody
- C76907 antibody
- FLJ20397 antibody
Immunocytochemistry/ Immunofluorescence - Anti-HEATR2 antibody (ab121622)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121622 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEATR2 antibody (ab121622)
ab121622 at 1/20 staining HEATR2 in Paraffin Embedded Human kidney tissue by Immunohistochemistry.
Note: strong cytoplasmic positivity in cells of tubules.
has not yet been referenced specifically in any publications.
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