Heat Mediated High pH Antigen Retrieving Solution (ab972)

Submit an Abreview Q&A (1)Specific References (1)

概述

  • 产品名称
    Heat Mediated High pH Antigen Retrieving Solution
    参阅全部 Antigen retrieval solution 试剂
  • 经测试应用
    适用于: IHC-Pmore details
  • 常规说明
    This antigen retrieval solution (pH 9.5) incorporates a colored pH indicator. The end-user is assured, by visual inspection, that the solution is at the correct dilution and pH. This product is specially formulated for superior pH stability at high temperatures, and will help prevent the possibility of losing pH sensitive antigens. When heating, the solution should turn gray. If the color of the solution turns yellow or red, it is an indication that the pH of the solution is incorrect and that staining may be hindered.


    This one-step high pH solution (9.5) allows the end-user to simultaneously deparaffinize and to heat-pretreat formalin-fixed paraffin-embedded tissues. Xylenes and alcohols are not needed for deparaffinization, thus saving valuable technician time and reduce costs for reagents and hazardous waste. Alternatively, tissues can be deparaffinized in xylene and hydrated to water, and then placed into the solution in the pressure cooker and/or other heating methods. This product also has blocking reagents to reduced non-specific background staining.

性能

  • 形式
    Liquid
  • 存放说明
    Store at room temperature.
  • 存储溶液
    pH: 9.50
    Preservative: Proprietary preservative
    Constituents: Carrier protein, PBS
    Note: Proprietary preservative
  • Concentration information loading...
  • Reagent说明
    This one-step high pH solution (9.5) allows the end-user to simultaneously deparaffinize and to heat-pretreat formalin-fixed paraffin-embedded tissues. Xylenes and alcohols are not needed for deparaffinization, thus saving valuable technician time and reduce costs for reagents and hazardous waste. Alternatively, tissues can be deparaffinized in xylene and hydrated to water, and then placed into the solution in the pressure cooker and/or other heating methods. This product also has blocking reagents to reduced non-specific background staining.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.

文献

This product has been referenced in:
  • Rosen D  et al. Accelerated thymic maturation and autoreactive T cells in bronchopulmonary dysplasia. Am J Respir Crit Care Med 174:75-83 (2006). IHC-P ; Baboon . Read more (PubMed: 16574933) »

See 1 Publication for this product

发表研究结果有使用 ab972？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

提交评价 提交问题

1 Review or Q&A

10
Votes: Highest First

Thank you for your answer. I received additional enquiries from the customer. 1. Can you inform us of the temperature of antigen retrieval solution at 2nd part of the procedure shown on the web? 2. Can you inform us of an appropriate time of heati...

Read More

Thank you for your enquiry. Our QC procedure is to heat the Borg up to 125 degrees for 30 second and then cool down to 90 degrees. This procedure takes place in our pressure cooker. The entire process takes 35 minutes. We have not tried it in an autocl...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册