RabMAb

Anti-HE4抗体[EPR4743] (ab109298)

概述

  • 产品名称Anti-HE4抗体[EPR4743]
    参阅全部 HE4 一抗
  • 描述
    兔单克隆抗体[EPR4743] to HE4
  • 经测试应用适用于: ICC/IF, WB, Flow Cyt, IPmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human HE4

  • 阳性对照
    • WB: HT29 cell lysates, SW480 cell lysates, Ovary cancer cell lysates, SK-OV-3 cell lysates. FC: permeabilized SW480 cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-HE4 antibody (HRP) [EPR4743] (ab198576)
    Anti-HE4 antibody (Alexa Fluor® 488) [EPR4743] (ab197885)
    Anti-HE4 antibody (Alexa Fluor® 647) [EPR4743] (ab197886)

     

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EPR4743
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab109298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 13 kDa).
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 组织特异性Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.
    • 序列相似性Contains 2 WAP domains.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 形式There are 5 isoforms produced by alternative splicing.
    • 别名
      • dJ461P17.6 antibody
      • EDDM4 antibody
      • epididymal protein 4 antibody
      • Epididymal secretory protein E4 antibody
      • Epididymis specific whey acidic protein type four disulfide core antibody
      • HE 4 antibody
      • Major epididymis specific protein E4 antibody
      • Major epididymis-specific protein E4 antibody
      • MGC57529 antibody
      • Putative protease inhibitor WAP5 antibody
      • WAP 5 antibody
      • WAP domain containing protein HE4 antibody
      • WAP domain containing protein HE4 V4 antibody
      • WAP four disulfide core domain 2 antibody
      • WAP four disulfide core domain protein 2 antibody
      • WAP four-disulfide core domain protein 2 antibody
      • WAP5 antibody
      • WFDC 2 antibody
      • WFDC2 antibody
      • WFDC2_HUMAN antibody
      see all

    Anti-HE4 antibody [EPR4743] 图像

    • All lanes : Anti-HE4 antibody [EPR4743] (ab109298) at 1/1000 dilution

      Lane 1 : HT29 cell lysate
      Lane 2 : SW480 cell lysate
      Lane 3 : Ovary cancer tissue lysate
      Lane 4 : SK-OV-3 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 13 kDa
      Observed band size : 23 kDa (why is the actual band size different from the predicted?)
    • Immunocytochemistry/Immunofluorescence analysis of SK-OV-3 (human ovarian adenocarcinoma) cells labelling HE4 with ab109298 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue). The negative control was SK-OV-3 cells stained with only DAPI, without ab109298.

      Confocal image showing cytoplasmic staining on SK-OV-3 cells.

    • ab109298, at 1/100 dilution, staining HE4 in permeabilized SW480 cells (red) and rabbit IgG as a negative control (green) using flow cytometry.

    Anti-HE4 antibody [EPR4743] (ab109298)参考文献

    This product has been referenced in:
    • Zhuang H  et al. Human epididymis protein 4 in association with Annexin II promotes invasion and metastasis of ovarian cancer cells. Mol Cancer 13:243 (2014). WB, IP ; Human . Read more (PubMed: 25362534) »
    • Lebleu VS  et al. Identification of human epididymis protein-4 as a fibroblast-derived mediator of fibrosis. Nat Med 19:227-31 (2013). Neutralising . Read more (PubMed: 23353556) »

    See all 3 Publications for this product

    Product Wall

    Thank you for contacting us.

    What application do you need the matched pair antibodies for? I assume it would be ELISA?

    None of the 5 HE4 antibodies have been tested together, thus we do not know if the ones you suggested (ab109298, ab...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (human pancreas and colon)
    Total protein in input 5000 µg
    Specification human pancreas and colon
    Immuno-precipitation step Protein G
    Username

    Mr. Qing Wang

    Verified customer

    提交于 Aug 12 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"