概述

  • 产品名称Anti-HE4抗体
    参阅全部 HE4 一抗
  • 描述
    兔多克隆抗体to HE4
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human HE4.

    (Peptide available as ab98946.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Human lung; Human ovary.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab85179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 13 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 组织特异性Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.
  • 序列相似性Contains 2 WAP domains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 形式There are 5 isoforms produced by alternative splicing.
  • 别名
    • dJ461P17.6 antibody
    • EDDM4 antibody
    • epididymal protein 4 antibody
    • Epididymal secretory protein E4 antibody
    • Epididymis specific whey acidic protein type four disulfide core antibody
    • HE 4 antibody
    • Major epididymis specific protein E4 antibody
    • Major epididymis-specific protein E4 antibody
    • MGC57529 antibody
    • Putative protease inhibitor WAP5 antibody
    • WAP 5 antibody
    • WAP domain containing protein HE4 antibody
    • WAP domain containing protein HE4 V4 antibody
    • WAP four disulfide core domain 2 antibody
    • WAP four disulfide core domain protein 2 antibody
    • WAP four-disulfide core domain protein 2 antibody
    • WAP5 antibody
    • WFDC 2 antibody
    • WFDC2 antibody
    • WFDC2_HUMAN antibody
    see all

Anti-HE4 antibody 图像

  • All lanes : Anti-HE4 antibody (ab85179) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human ovary tissue lysate - total protein (ab30222)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 13 kDa
    Observed band size : 14 kDa (why is the actual band size different from the predicted?)


    Exposure time : 16 minutes
  • ICC/IF image of ab85179 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85179, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 1µg/ml.

Anti-HE4 antibody (ab85179)参考文献

This product has been referenced in:
  • Markovic-Lipkovski J  et al. Variable Expression of Neural Cell Adhesion Molecule Isoforms in Renal Tissue: Possible Role in Incipient Renal Fibrosis. PLoS One 10:e0137028 (2015). ICC/IF ; Human . Read more (PubMed: 26327314) »

See 1 Publication for this product

Product Wall

Abreviews
Application Western blot
Loading amount 20 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (15 % Tris-HCl criterion precast gel (Biorad))
Sample Horse Cell lysate - other (equine concentrated oviductal flush)
Specification equine concentrated oviductal flush
Treatment concentrated (centrivap) + resuspended
Blocking step Tween-20 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.3% · Temperature: 18°C
Username

Ms. Hilde Nelis

Verified customer

提交于 Oct 25 2013

Thank you for contacting us.

What application do you need the matched pair antibodies for? I assume it would be ELISA?

None of the 5 HE4 antibodies have been tested together, thus we do not know if the ones you suggested (ab109298, ab...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"