Customer reviews and FAQs

概述

  • 产品名称Anti-HE4抗体
    参阅全部 HE4 一抗
  • 描述
    兔多克隆抗体to HE4
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human mature HE4 fused to glutathione S-transferase. Produced in E.coli.

  • 阳性对照
    • Normal human epididymis or human ovarian tumour.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, BSA
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab24480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100. Detects a band of approximately 25 kDa (predicted molecular weight: 13 kDa).
IHC-P 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 组织特异性Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.
  • 序列相似性Contains 2 WAP domains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
    • 形式There are 5 isoforms produced by alternative splicing.
    • 别名
      • dJ461P17.6 antibody
      • EDDM4 antibody
      • epididymal protein 4 antibody
      • Epididymal secretory protein E4 antibody
      • Epididymis specific whey acidic protein type four disulfide core antibody
      • HE 4 antibody
      • Major epididymis specific protein E4 antibody
      • Major epididymis-specific protein E4 antibody
      • MGC57529 antibody
      • Putative protease inhibitor WAP5 antibody
      • WAP 5 antibody
      • WAP domain containing protein HE4 antibody
      • WAP domain containing protein HE4 V4 antibody
      • WAP four disulfide core domain 2 antibody
      • WAP four disulfide core domain protein 2 antibody
      • WAP four-disulfide core domain protein 2 antibody
      • WAP5 antibody
      • WFDC 2 antibody
      • WFDC2 antibody
      • WFDC2_HUMAN antibody
      see all

    Anti-HE4 antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HE4 antibody (ab24480)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HE4 antibody (ab24480)
      ab24480 (4µg/ml) staining HE4 in human lung using an automated system (DAKO Autostainer Plus).
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    Anti-HE4 antibody (ab24480)参考文献

    This product has been referenced in:
    • Markovic-Lipkovski J  et al. Variable Expression of Neural Cell Adhesion Molecule Isoforms in Renal Tissue: Possible Role in Incipient Renal Fibrosis. PLoS One 10:e0137028 (2015). IHC-P ; Human . Read more (PubMed: 26327314) »
    • Tokuishi K  et al. Splice variant HE4-V3 expression is associated with favorable prognosis in pulmonary adenocarcinoma. Tumour Biol 33:103-9 (2012). WB, IHC-P ; Human . Read more (PubMed: 22015929) »

    See all 4 Publications for this product

    发表研究结果有使用 ab24480？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    Which isoform does this antibody detect?

    The antibody is a polyclonal that was raised against a GST-fusion of the full length HE4 protein. As a result, it is likely to recognize all isoforms, but this has not been specifically tested.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"