- 数据表
- 具体的参考文献 (7)
- 实验方案
概述
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产品名称Anti-HE4抗体
参阅全部 HE4 一抗 -
描述兔多克隆抗体to HE4
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宿主Rabbit
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经测试应用适用于: WB, IHC-Pmore details
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种属反应性与反应: Human
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免疫原
Human mature HE4 fused to glutathione S-transferase. Produced in E.coli.
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阳性对照
- Normal human epididymis or human ovarian tumour.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.1% Sodium Azide
Constituents: PBS, BSA -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunohistochemistry kits
应用
Our Abpromise guarantee covers the use of ab24480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/100. Detects a band of approximately 25 kDa (predicted molecular weight: 13 kDa). | |
| IHC-P | 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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组织特异性Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.
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序列相似性Contains 2 WAP domains.
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细胞定位Secreted.
- Information by UniProt
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数据库链接
- Entrez Gene: 10406 Human
- SwissProt: Q14508 Human
- Unigene: 2719 Human
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形式There are 5 isoforms produced by alternative splicing.
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别名
- dJ461P17.6 antibody
- EDDM4 antibody
- epididymal protein 4 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HE4 antibody (ab24480)ab24480 (4µg/ml) staining HE4 in human lung using an automated system (DAKO Autostainer Plus).
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
文献
This product has been referenced in:
- Shi LP et al. MicroRNA-149 sensitizes colorectal cancer to radiotherapy by downregulating human epididymis protein 4. Am J Cancer Res 8:30-38 (2018). Read more (PubMed: 29416918) »
- Owen GI et al. Chilean Gastric Cancer Task Force: A study protocol to obtain a clinical and molecular classification of a cohort of gastric cancer patients. Medicine (Baltimore) 97:e0419 (2018). Read more (PubMed: 29668600) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"