概述

  • 产品名称
    Anti-HDLBP抗体[EPR5267]
    参阅全部 HDLBP 一抗
  • 描述
    兔单克隆抗体[EPR5267] to HDLBP
  • 经测试应用
    适用于: ICC/IF, WB, IHC-Pmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human HDLBP.

  • 阳性对照
    • WB: 293T, Jurkat, HeLa and HepG2 cell lysates IHC-P: Human stomach tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

应用

Our Abpromise guarantee covers the use of ab109324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500.
WB 1/10000 - 1/50000. Detects a band of approximately 155 kDa (predicted molecular weight: 141 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 应用说明
    Is unsuitable for Flow Cyt or IP.
  • 靶标

    图片

    • All lanes : Anti-HDLBP antibody [EPR5267] (ab109324) at 1/10000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 141 kDa
      Observed band size : 155 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescence staining of HeLa cells with purified ab109324 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

    • ab109324, at a 1/100 dilution, staining HDLBP in paraffin embedded Human stomach tissue by Immunohistochemistry.

    文献

    This product has been referenced in:
    • Mobin MB  et al. The RNA-binding protein vigilin regulates VLDL secretion through modulation of Apob mRNA translation. Nat Commun 7:12848 (2016). Read more (PubMed: 27665711) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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