概述

  • 产品名称Anti-HDGF抗体[26C4a]
    参阅全部 HDGF 一抗
  • 描述
    小鼠单克隆抗体[26C4a] to HDGF
  • 经测试应用适用于: WB, Dot blot, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment of human HDGF, from an internal sequence.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明ab58644 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • 克隆单克隆
  • 克隆编号26C4a
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab58644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 27 kDa).
Dot blot Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 21960592

靶标

  • 功能Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the HDGF family.
    Contains 1 PWWP domain.
  • 结构域The PWWP domain harbors the heparin-binding sites and is responsible for DNA-binding, while the C-terminal region is essentially unstructured.
  • 翻译后修饰Sumoylated by SUMO1. Sumoylation prevents binding to chromatin.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ96580 antibody
    • HDGF antibody
    • HDGF_HUMAN antibody
    • Hepatoma derived growth factor antibody
    • Hepatoma-derived growth factor antibody
    • High mobility group protein 1 like 2 antibody
    • High mobility group protein 1-like 2 antibody
    • HMG-1L2 antibody
    • HMG1L2 antibody
    see all

Anti-HDGF antibody [26C4a] 图像

  • Anti-HDGF antibody [26C4a] (ab58644) at 1/100 dilution + immunising recombinant protein at 25 µg

    Secondary
    Sheep Anti Mouse IgG at 1/3000 dilution

    Predicted band size : 27 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

Anti-HDGF antibody [26C4a] (ab58644)参考文献

This product has been referenced in:
  • Leich E  et al. MicroRNA profiles of t(14;18)-negative follicular lymphoma support a late germinal center B-cell phenotype. Blood 118:5550-8 (2011). IHC-P ; Human . Read more (PubMed: 21960592) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"