Anti-HDC2 / PHC2抗体(ab124405)

概述

  • 产品名称
    Anti-HDC2 / PHC2抗体
  • 描述
    兔多克隆抗体to HDC2 / PHC2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant fragment, corresponding to amino acids 553-835 of Human HDC2/ PHC2 (BC029269; UniProt ID: Q8IXK0).

  • 阳性对照
    • HEK293 and MCF7 lysates

性能

应用

Our Abpromise guarantee covers the use of ab124405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 36, 30 kDa (predicted molecular weight: 91,88,36,30 kDa).

靶标

  • 功能
    Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
  • 序列相似性
    Contains 1 FCS-type zinc finger.
    Contains 1 SAM (sterile alpha motif) domain.
  • 结构域
    HD1 motif interacts with SAM domain of PHC1.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 形式
    There are 5 isoforms produced by alternative splicing.
  • 别名
    • EDR2 antibody
    • early development regulator 2 (homolog of polyhomeotic 2) antibody
    • early development regulator 2 like antibody
    • Early development regulatory protein 2 antibody
    • hPH2 antibody
    • MGC163502 antibody
    • mph2 antibody
    • PH2 antibody
    • PHC2 antibody
    • PHC2_HUMAN antibody
    • polyhomeotic 2 antibody
    • polyhomeotic like 2 antibody
    • Polyhomeotic-like protein 2 antibody
    see all

图片

  • All lanes : Anti-HDC2 / PHC2 antibody (ab124405) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : MCF7 cell lysate


    Predicted band size : 91,88,36,30 kDa
    Observed band size : 30,36 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab124405 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic stem cells)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
50 µg
Treatment
2i and LIF
Specification
Mouse embryonic stem cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 24°C
Username

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提交于 Sep 15 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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