概述

  • 产品名称Anti-HDAC9抗体[EPR5223]
    参阅全部 HDAC9 一抗
  • 描述
    兔单克隆抗体[EPR5223] to HDAC9
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of Human HDAC9.

  • 阳性对照
    • K562, HepG2, and Raji cell lysates; Human colon tissue; HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-HDAC9 antibody (Alexa Fluor® 488) [EPR5223] (ab206713)

    Anti-HDAC9 antibody (Alexa Fluor® 647) [EPR5223] (ab207050)

性能

应用

Our Abpromise guarantee covers the use of ab109446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 160 kDa (predicted molecular weight: 111 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.
      Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
    • 组织特异性Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).
    • 疾病相关Note=A chromosomal aberration involving HDAC9 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with TGFB2 resulting in lack of HDAC9 protein.
    • 序列相似性Belongs to the histone deacetylase family. HD type 2 subfamily.
    • 翻译后修饰Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
      Sumoylated.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • HD 7 antibody
      • HD 7B antibody
      • HD 9 antibody
      • HD7 antibody
      • HD7B antibody
      • HD9 antibody
      • HDAC 7 antibody
      • HDAC 7B antibody
      • HDAC 9 antibody
      • HDAC 9B antibody
      • HDAC 9FL antibody
      • HDAC antibody
      • HDAC7 antibody
      • HDAC7B antibody
      • HDAC9 antibody
      • HDAC9_HUMAN antibody
      • HDAC9B antibody
      • HDAC9FL antibody
      • HDRP antibody
      • Histone deacetylase 4/5 related protein antibody
      • Histone deacetylase 7 antibody
      • Histone deacetylase 7B antibody
      • Histone deacetylase 9 antibody
      • Histone deacetylase 9A antibody
      • Histone deacetylase related protein antibody
      • Histone deacetylase-related protein antibody
      • KIAA0744 antibody
      • MEF2 interacting transcription repressor MITR antibody
      • MEF2 interacting transcription repressor protein antibody
      • MEF2-interacting transcription repressor MITR antibody
      • MITR antibody
      see all

    Anti-HDAC9 antibody [EPR5223] 图像

    • All lanes : Anti-HDAC9 antibody [EPR5223] (ab109446) at 1/10000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 111 kDa
      Observed band size : 160 kDa (why is the actual band size different from the predicted?)
    • ab109446 at 1/250 dilution staining HDAC9 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.
    • ab109446 at 1/100 dilution staining HDAC9 in HeLa cells by Immunofluorescence.

    Anti-HDAC9 antibody [EPR5223] (ab109446)参考文献

    This product has been referenced in:
    • Sebastian S  et al. Tissue-specific splicing of a ubiquitously expressed transcription factor is essential for muscle differentiation. Genes Dev 27:1247-59 (2013). Read more (PubMed: 23723416) »
    • Klinz FJ  et al. Histone deacetylases 2 and 9 are coexpressed and nuclear localized in human molar odontoblasts in vivo. Histochem Cell Biol : (2012). ICC/IF, IHC-FrFl ; Human . Read more (PubMed: 22297573) »

    See all 2 Publications for this product

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