Anti-HDAC9抗体- ChIP Grade (ab59718)

概述

  • 产品名称Anti-HDAC9抗体- ChIP Grade
    参阅全部 HDAC9 一抗
  • 描述
    兔多克隆抗体to HDAC9 - ChIP Grade
  • 特异性This antibody recognises Histone deacetylase 9, isoform CRA b/isoform 3, also known as HDRP.
  • 经测试应用适用于: WB, IHC-P, ChIP, ELISA, IHC-Frmore details
  • 种属反应性
    与反应: Human
    预测可用于: Sheep, Chicken, Guinea pig, Dog
  • 免疫原

    A region within synthetic peptide: QVGAVKVKEE PVDSDEDAQI QEMESGEQAA FMQQVIGKDL APGFVIKVII, corresponding to C terminal amino acids 541-590 of Human HDAC9

  • 阳性对照
    • A172 cell lysate or human kidney tissue.

性能

应用

Our Abpromise guarantee covers the use of ab59718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 66 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ChIP Use at an assay dependent concentration. PubMed: 20596014
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 25011550

靶标

  • 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.
    Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
  • 组织特异性Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).
  • 疾病相关Note=A chromosomal aberration involving HDAC9 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with TGFB2 resulting in lack of HDAC9 protein.
  • 序列相似性Belongs to the histone deacetylase family. HD type 2 subfamily.
  • 翻译后修饰Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    Sumoylated.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HD 7 antibody
    • HD 7B antibody
    • HD 9 antibody
    • HD7 antibody
    • HD7B antibody
    • HD9 antibody
    • HDAC 7 antibody
    • HDAC 7B antibody
    • HDAC 9 antibody
    • HDAC 9B antibody
    • HDAC 9FL antibody
    • HDAC antibody
    • HDAC7 antibody
    • HDAC7B antibody
    • HDAC9 antibody
    • HDAC9_HUMAN antibody
    • HDAC9B antibody
    • HDAC9FL antibody
    • HDRP antibody
    • Histone deacetylase 4/5 related protein antibody
    • Histone deacetylase 7 antibody
    • Histone deacetylase 7B antibody
    • Histone deacetylase 9 antibody
    • Histone deacetylase 9A antibody
    • Histone deacetylase related protein antibody
    • Histone deacetylase-related protein antibody
    • KIAA0744 antibody
    • MEF2 interacting transcription repressor MITR antibody
    • MEF2 interacting transcription repressor protein antibody
    • MEF2-interacting transcription repressor MITR antibody
    • MITR antibody
    see all

Anti-HDAC9 antibody - ChIP Grade 图像

  • ChIP analysis using ab59718 (0.5µg/mL) binding HDAC9 in human HCT116 colon cancer cells. Without (- peptide) and with (+ peptide) blocking peptide. Protein binding was detected using real-time PCR.

  • Anti-HDAC9 antibody - ChIP Grade (ab59718) at 1.25 µg/ml + A172 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG used at 1/50000

    Predicted band size : 66 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling HDAC9 with ab59718 at 4µg/ml. Epithelial cells of the renal tubule were positively labelled (indicated with arrows).

Anti-HDAC9 antibody - ChIP Grade (ab59718)参考文献

This product has been referenced in:
  • Shen W  et al. Epigenetic modification of the leptin promoter in diet-induced obese mice and the effects of N-3 polyunsaturated Fatty acids. Sci Rep 4:5282 (2014). ChIP ; Mouse . Read more (PubMed: 24923522) »
  • Tiwari S  et al. Histone deacetylase expression patterns in developing murine optic nerve. BMC Dev Biol 14:30 (2014). WB, IHC-Fr ; Mouse . Read more (PubMed: 25011550) »

See all 6 Publications for this product

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - BD permeabilization buffer
Sample Mouse Cell (Mouse thymus tissue, thymocytes)
Specification Mouse thymus tissue, thymocytes
Gating Strategy CD45 positives
Preparation Cell harvesting/tissue preparation method: Digestion with Collagenase D
Sample buffer: PBS 2% FCS
Username

Mr. Sanjay Gawade

Verified customer

提交于 Jan 26 2015

Thank you for contacting us. I have checked: The immunogen peptide should be available for sale. I am waiting to hear back from the lab what the catalog number and the price will be. Thank you for your patience. The immunogen is a region within synthet...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Cardiomyocytes)
Loading amount 10 µg
Specification Mouse Cardiomyocytes
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Prof. Paul Townsend

Verified customer

提交于 Oct 18 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"