HDAC8 Activity Assay试剂盒(Fluorometric) (ab156069)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture extracts, Tissue Extracts
概述
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产品名称
HDAC8 Activity Assay试剂盒(Fluorometric) -
检测方法
Fluorescent -
样品类型
Cell culture extracts, Tissue Extracts -
检测类型
Enzyme activity -
种属反应性
与反应: Human -
产品概述
Abcam’s HDAC8 Activity Assay Kit (Fluorometric) (ab156069) detects HDAC activity in lysates.
Primarily, the HDAC8 Activity Assay Kit (Fluorometric) is designed for the rapid and sensitive evaluation of HDAC inhibitors using recombinant HDAC8. Additionally, any cultured primary cell, cell line, or tissue homogenate can be assayed for HDAC8 activity with the HDAC8 Activity Assay Kit (Fluorometric) after immunoprecipitation with an appropriate HDAC8 specific antibody.
Applications for this kit include:
1. Monitoring the purification of HDACs including HDAC1, 2, 3 and 8 (class I).
2. Screening inhibitors or activators of HDAC8.
3. Detecting the effects of pharmacological agents on HDAC8.
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说明
Histone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), allowing the histones to wrap the DNA more tightly.
HDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity.
Classes I, IIA and IIB are considered "classical" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others. -
平台
Microplate reader
性能
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存放说明
Please refer to protocols. -
组件 100 tests Developer 1 x 500µl Fluoro-Deacetylated Peptide (0.2 mM) 1 x 100µl Fluoro-Substrate Peptide (0.2 mM) 1 x 500µl HDAC Assay Buffer 2 x 1ml Recombinant HDAC8 1 x 500µl Stop Solution 2 x 1ml Trichostatin A (200µM) 1 x 500µl -
研究领域
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功能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility. -
组织特异性
Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney. -
序列相似性
Belongs to the histone deacetylase family. HD type 1 subfamily. -
翻译后修饰
Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4. -
细胞定位
Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation. - Information by UniProt
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别名
- HD8
- HDAC 8
- Hdac8
see all -
数据库链接
- Entrez Gene: 55869 Human
- SwissProt: Q9BY41 Human
- Unigene: 310536 Human
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab156069 尚未被引用在任何文献中。