Anti-HDAC7 (phospho S155)抗体(ab111390)


  • 产品名称Anti-HDAC7 (phospho S155)抗体
    参阅全部 HDAC7 一抗
  • 描述
    兔多克隆抗体to HDAC7 (phospho S155)
  • 特异性ab111390 detects endogenous levels of HDAC7A only when phosphorylated at serine 155.
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human HDAC7A around the phosphorylation site of Serine 155 (T-V-Sp-E-P).

  • 阳性对照
    • HeLa cell extracts. IF/ICC: HepG2 cell line.




Our Abpromise guarantee covers the use of ab111390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 103 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.


  • 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene.
  • 序列相似性Belongs to the histone deacetylase family. HD type 2 subfamily.
  • 结构域The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • 翻译后修饰May be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
  • 细胞定位Nucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZP586J0917 antibody
    • FLJ99588 antibody
    • HD 7a antibody
    • HD7 antibody
    • HD7a antibody
    • HDAC 7 antibody
    • HDAC 7A antibody
    • Hdac7 antibody
    • HDAC7_HUMAN antibody
    • HDAC7A antibody
    • Histone deacetylase 7 antibody
    • Histone deacetylase 7A antibody
    • OTTHUMP00000202813 antibody
    • OTTHUMP00000202814 antibody
    see all

Anti-HDAC7 (phospho S155) antibody 图像

  • ICC/IF image of ab111390 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab111390, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-HDAC7 (phospho S155) antibody (ab111390) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with immunizing phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 103 kDa

Anti-HDAC7 (phospho S155) antibody (ab111390)参考文献

ab111390 has not yet been referenced specifically in any publications.

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