概述

  • 产品名称Anti-HDAC7抗体
    参阅全部 HDAC7 一抗
  • 描述
    兔多克隆抗体to HDAC7
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    YRTLEPLDTEGAARSVLSS

    with N-terminal added lysine, conjugated to KLH, corresponding to amino acids 137-155 of Mouse HDAC7. The corresponding sequence is identical in rat and differs by three amino acids in human.

  • 阳性对照
    • Mouse NIH-3T3 cells, whole extracts of rat PC12 cells (Western blotting)
  • 常规说明Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

性能

应用

Our Abpromise guarantee covers the use of ab12175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Predicted molecular weight: 105 kDa. Additional weak bands may be detected in various extract preparations. A minimum working antibody dilution of 1/2000 is recommended using whole extracts of mouse NIH-3T3 cells. A minimum working antibody dilution of 1/500 is recommended using whole extracts of rat PC12 cells.
IP Use at an assay dependent dilution. 1.5-1.8 µg of the antibody immunoprecipitates HDAC7 from a RIPA extract (180 µg) of 293T human embryonic kidney cells expressing recombinant human HDAC7.

靶标

  • 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene.
  • 序列相似性Belongs to the histone deacetylase family. HD type 2 subfamily.
  • 结构域The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • 翻译后修饰May be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
  • 细胞定位Nucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZP586J0917 antibody
    • FLJ99588 antibody
    • HD 7a antibody
    • HD7 antibody
    • HD7a antibody
    • HDAC 7 antibody
    • HDAC 7A antibody
    • Hdac7 antibody
    • HDAC7_HUMAN antibody
    • HDAC7A antibody
    • Histone deacetylase 7 antibody
    • Histone deacetylase 7A antibody
    • OTTHUMP00000202813 antibody
    • OTTHUMP00000202814 antibody
    see all

Anti-HDAC7 antibody (ab12175)参考文献

This product has been referenced in:
  • Jensen ED  et al. Bone morphogenic protein 2 activates protein kinase D to regulate histone deacetylase 7 localization and repression of Runx2. J Biol Chem 284:2225-34 (2009). IP ; Mouse . Read more (PubMed: 19029091) »

See 1 Publication for this product

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