The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Predicted molecular weight: 105 kDa. Additional weak bands may be detected in various extract preparations. A minimum working antibody dilution of 1/2000 is recommended using whole extracts of mouse NIH-3T3 cells. A minimum working antibody dilution of 1/500 is recommended using whole extracts of rat PC12 cells.
Use at an assay dependent dilution. 1.5-1.8 µg of the antibody immunoprecipitates HDAC7 from a RIPA extract (180 µg) of 293T human embryonic kidney cells expressing recombinant human HDAC7.
功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene.
序列相似性Belongs to the histone deacetylase family. HD type 2 subfamily.
结构域The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
翻译后修饰May be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
细胞定位Nucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation.
Jensen ED et al. Bone morphogenic protein 2 activates protein kinase D to regulate histone deacetylase 7 localization and repression of Runx2. J Biol Chem284:2225-34 (2009).
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