HDAC6 overexpression 293T裂解液(全细胞) (ab94173)

概述

  • 产品名称HDAC6 overexpression 293T裂解液(全细胞)
  • 描述
    HDAC6 overexpression裂解液
  • 常规说明ab94173 is a 293T cell transfected lysate in which Human HDAC6 has been transiently over-expressed using a pCMV-HDAC6 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Similarity: Belongs to the histone deacetylase family. HD type 2 subfamily. Contains 1 UBP-type zinc finger. PTM: Ubiquitinated. Its polyubiquitination however does not lead to its degradation. Sumoylated in vitro.

应用

Our Abpromise guarantee covers the use of ab94173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

HDAC6 overexpression 293T lysate (whole cell) 图像

  • ab94173 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-HDAC6 antibody (ab56926) at 1/500 dilution

    Lane 1 : HDAC6 overexpression 293T lysate (whole cell) (ab94173)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

HDAC6 overexpression 293T lysate (whole cell) (ab94173)参考文献

ab94173 has not yet been referenced specifically in any publications.

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