概述

  • 产品名称Anti-HDAC6抗体
    参阅全部 HDAC6 一抗
  • 描述
    兔多克隆抗体to HDAC6
  • 特异性The antibody detects ~134 kDa histone deacetylase 6. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 5, 7, and 8.
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Mouse) - N terminal.

  • 阳性对照
    • mouse small intestine lysate, jurkat cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab1440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 131 kDa).Can be blocked with HDAC6 peptide (ab41708). Block with 5% milk in TBST and incubate the antibody in blocking buffer.

靶标

  • 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
  • 序列相似性Belongs to the histone deacetylase family. HD type 2 subfamily.
    Contains 1 UBP-type zinc finger.
  • 翻译后修饰Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
    Sumoylated in vitro.
  • 细胞定位Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CPBHM antibody
    • FLJ16239 antibody
    • HD 6 antibody
    • HD6 antibody
    • HDAC 6 antibody
    • HDAC6 antibody
    • HDAC6_HUMAN antibody
    • Histone deacetylase 6 (HD6) antibody
    • Histone deacetylase 6 antibody
    • JM 21 antibody
    • JM21 antibody
    • KIAA0901 antibody
    • OTTHUMP00000032398 antibody
    • OTTHUMP00000197663 antibody
    • PPP1R90 antibody
    • Protein phosphatase 1 regulatory subunit 90 antibody
    see all

Anti-HDAC6 antibody 图像

  • Anti-HDAC6 antibody (ab1440) + Jurkat cell lysate

    Predicted band size : 131 kDa
    Observed band size : 131 kDa

Anti-HDAC6 antibody (ab1440)参考文献

This product has been referenced in:

See all 8 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 28°C
Sample Mouse Cell (Neural cell)
Specification Neural cell
Permeabilization Yes - 0.3% TritonX-100 in PBS for 30 minutes
Fixative Paraformaldehyde
Username

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提交于 Oct 20 2014

Abreviews
Application Western blot
Sample Rat Cell lysate - whole cell (Primary Neurons)
Loading amount 20 µg
Specification Primary Neurons
Gel Running Conditions Reduced Denaturing (4-12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 May 15 2013

Thank you for your reply.

I looked up the order associated with ab1440, and it look as though you received that product for free as part of our 4for3 promotion a month ago. Since you received it for free, there is no cost to refund, but I am ...

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Thank you for providing that image.

Based on the information you have provided, I do beleive that there a re protocol varations that I can suggest that will help resolve this situation, therefore I would be happy to replace or refund the cost...

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Thank you for contacting Abcam.

I am sorry that you are having some issues with ab1440. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human samples . If we cannot resolve the issue you...

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Thank you for your enquiry. I have been in touch with our lab team and they have informed me that for the application of this antibody by IHC using paraffin embedded sections antigen retrieval should be performed by heating sections in a microwave ...

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Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab1440. This antibody has been tested with HeLa cell lysate (20 ug) and a ~134 kDa band was detected. The antibody should with mouse samples as well. Can you tel...

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The immunogen used was a synthetic peptide mapping to Mouse HDAC-6 N-terminus. If you have any additional questions, please contact us again.

Thank you for the information. I have instructed our accounting department to issue you a refund for ab1440 and ab1437. This process does take a little while and if you have any questions regarding the refund, please contact our accounts department at ...

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Thank you for the information regarding ab1440. I searched through our system and I don't have a record of an enquiry from you regarding ab1437. Can you please send me a description of the problem with ab1437 and your protocol? I will then investigate ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"