Anti-HDAC6抗体(ab117516)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to HDAC6
  • 经测试应用
    适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to amino acids in Human HDAC6 (NP_006035.2).

  • 阳性对照
    • Human intestine (ganglion cells) and Human testis tissues; HepG2 cells.

性能

应用

Our Abpromise guarantee covers the use of ab117516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 131 kDa.
ELISA 1/1000.
IHC-P 1/200.
ICC/IF 1/100 - 1/500.

靶标

  • 功能
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
  • 序列相似性
    Belongs to the histone deacetylase family. HD type 2 subfamily.
    Contains 1 UBP-type zinc finger.
  • 翻译后修饰
    Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
    Sumoylated in vitro.
  • 细胞定位
    Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CPBHM antibody
    • FLJ16239 antibody
    • HD 6 antibody
    • HD6 antibody
    • HDAC 6 antibody
    • HDAC6 antibody
    • HDAC6_HUMAN antibody
    • Histone deacetylase 6 (HD6) antibody
    • Histone deacetylase 6 antibody
    • JM 21 antibody
    • JM21 antibody
    • KIAA0901 antibody
    • OTTHUMP00000032398 antibody
    • OTTHUMP00000197663 antibody
    • PPP1R90 antibody
    • Protein phosphatase 1 regulatory subunit 90 antibody
    see all

Anti-HDAC6 antibody 图像

  • ab117516, at 1:200 dilution, staining HDAC6 in Formalin-fixed, Paraffin-embedded Human intestine tissue (ganglion cells) by Immunohistochemistry.

  • ab117516, at 1:200 dilution, staining HDAC6 in Formalin-fixed, Paraffin-embedded Human testis tissue by Immunohistochemistry.

  • All lanes : Anti-HDAC6 antibody (ab117516) at 1/500 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with immunizing peptide


    Predicted band size : 131 kDa
  • ab117516, at 1/100, staining HDAC6 in HepG2 cells by Immunofluorescence (Left image).
    The image on the right is treated with the synthesized peptide.

Anti-HDAC6 antibody (ab117516)参考文献

ab117516 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Sep 26 2017

Application
Western blot
Sample
Human Cell lysate - nuclear (LNCaP - Prostate Cancer Cells)
Gel Running Conditions
Reduced Denaturing (4-15% SDS PAGE)
Loading amount
35 µg
Specification
LNCaP - Prostate Cancer Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 May 09 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP - prostate cancer cells)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
LNCaP - prostate cancer cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Apr 07 2017

Thank you for your enquiry. I am sorry to confirm that as far as we are aware,  ab117516  Anti-HDAC6 antibody has not been tested and is therefore not guaranteed in mouse. I would be very pleased to hear to provide you with a discount code for the cust...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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