Anti-HDAC5 (phospho S498)抗体(ab47283)

概述

  • 产品名称Anti-HDAC5 (phospho S498)抗体
    参阅全部 HDAC5 一抗
  • 描述
    兔多克隆抗体to HDAC5 (phospho S498)
  • 特异性This antibody is specific for HDAC5, only when phosphorylated at Serine 498.
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human HDAC5 (phospho S498). Immunogen range: 464-513
    Database link: Q9UQL6

  • 阳性对照
    • NIH/3T3 cell extracts; Breast carcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab47283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 118 kDa (predicted molecular weight: 122 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

靶标

  • 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the histone deacetylase family. HD type 2 subfamily.
  • 结构域The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • 翻译后修饰Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    Ubiquitinated. Polyubiquitination however does not lead to its degradation.
  • 细胞定位Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Antigen NY CO 9 antibody
    • Antigen NY-CO-9 antibody
    • HD5 antibody
    • HDAC 5 antibody
    • HDAC5 antibody
    • HDAC5_HUMAN antibody
    • Histone deacetylase 5 antibody
    • NY CO 9 antibody
    see all

Anti-HDAC5 (phospho S498) antibody 图像

  • All lanes : Anti-HDAC5 (phospho S498) antibody (ab47283) at 1/500 dilution

    Lane 1 : Extracts from NIH/ 3T3 cells
    Lane 2 : Extracts from NIH/ 3T3 cells; ab47283 preincubated with immunising peptide (blocking)


    Predicted band size : 122 kDa
    Observed band size : 118 kDa (why is the actual band size different from the predicted?)
  • ab47283 (1/100) staining HDAC5 (phospho S498) in paraffin embedded human breast carcinoma tissue. For the right hand panel, ab47283 was pre-incubated with the immunizing peptide (blocking).

Anti-HDAC5 (phospho S498) antibody (ab47283)参考文献

This product has been referenced in:
  • Yuan SY  et al. AMPK Mediates Glucocorticoids Stress-Induced Downregulation of the Glucocorticoid Receptor in Cultured Rat Prefrontal Cortical Astrocytes. PLoS One 11:e0159513 (2016). Rat . Read more (PubMed: 27513844) »
  • Mitsi V  et al. RGS9-2--controlled adaptations in the striatum determine the onset of action and efficacy of antidepressants in neuropathic pain states. Proc Natl Acad Sci U S A 112:E5088-97 (2015). WB ; Mouse . Read more (PubMed: 26305935) »

See all 8 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (5%)
Sample Rat Cell lysate - whole cell (Hippocampal primary Neurons)
Specification Hippocampal primary Neurons
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Nov 24 2014

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