概述

  • 产品名称Anti-HDAC5抗体[HDAC5-35]
    参阅全部 HDAC5 一抗
  • 描述
    小鼠单克隆抗体[HDAC5-35] to HDAC5
  • 经测试应用适用于: ICC/IF, ELISA, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human HDAC5 aa 4-19 conjugated to keyhole limpet haemocyanin. synthetic peptide has a C-terminal added lysine.
    Sequence:

    PNESDGMSGREPSLEI


    Database link: Q9UQL6

  • 阳性对照
    • WB: Total cell extracts of NIH3T3 fibroblast cells.

性能

应用

Our Abpromise guarantee covers the use of ab50001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 122 kDa.

靶标

  • 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the histone deacetylase family. HD type 2 subfamily.
  • 结构域The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • 翻译后修饰Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    Ubiquitinated. Polyubiquitination however does not lead to its degradation.
  • 细胞定位Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Antigen NY CO 9 antibody
    • Antigen NY-CO-9 antibody
    • HD5 antibody
    • HDAC 5 antibody
    • HDAC5 antibody
    • HDAC5_HUMAN antibody
    • Histone deacetylase 5 antibody
    • NY CO 9 antibody
    see all

Anti-HDAC5 antibody [HDAC5-35] 图像

  • Lane 1 : Anti-HDAC5 antibody [HDAC5-35] (ab50001) at 2 µg/ml
    Lane 2 : no primary antibody

    Lane 1 : 3T3 cell extract (mouse fibroblasts)
    Lane 2 : 3T3 cell extract (mouse fibroblasts)

    Secondary
    Goat Anti-Mouse, Peroxidase conjugate

    Predicted band size : 122 kDa
    Observed band size : ~122 kDa (why is the actual band size different from the predicted?)

Anti-HDAC5 antibody [HDAC5-35] (ab50001)参考文献

This product has been referenced in:
  • Mitsi V  et al. RGS9-2--controlled adaptations in the striatum determine the onset of action and efficacy of antidepressants in neuropathic pain states. Proc Natl Acad Sci U S A 112:E5088-97 (2015). WB ; Mouse . Read more (PubMed: 26305935) »

See 1 Publication for this product

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