概述

  • 产品名称
  • 描述
    兔多克隆抗体to HDAC5
  • 特异性
    The antibody detects ~124 kDa histone deacetylase 5. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 6, 7, and 8.
  • 经测试应用
    适用于: IHC-FoFr, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human HDAC5. The exact sequence is proprietary. Synthetic peptide surrounding amino acid 575
    Database link: Q9UQL6

  • 阳性对照
    • WB: HeLa, HepG2 and Jurkay cell lysates; rat hippocampus tissue lysate.
  • 常规说明
    Some tissues may not express the HDAC-4 and 5 protein or it is expressed at extremely low amount. HDAC-1,2,3,6,7 are usually much more abundant (could be ~100X more) than HDAC-4 & 5. We would strongly suggest using positive control (HeLa lysate).

性能

应用

Our Abpromise guarantee covers the use of ab1439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 19939859
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 121.9 kDa.

HDAC-5 is in very low abundance (~100X lower) in comparison to other HDACs (e.g., HDAC-1, 2, 6, or 7, etc.). For certain tissue and cells, it can be very difficult to detect due to the low abundance, we recommend to try to use strong lysis buffers (containing NP40),load more protein and use more antibody.

靶标

  • 功能
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
  • 组织特异性
    Ubiquitous.
  • 序列相似性
    Belongs to the histone deacetylase family. HD type 2 subfamily.
  • 结构域
    The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • 翻译后修饰
    Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    Ubiquitinated. Polyubiquitination however does not lead to its degradation.
  • 细胞定位
    Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Antigen NY CO 9 antibody
    • Antigen NY-CO-9 antibody
    • HD5 antibody
    • HDAC 5 antibody
    • HDAC5 antibody
    • HDAC5_HUMAN antibody
    • Histone deacetylase 5 antibody
    • NY CO 9 antibody
    see all

图片

  • Anti-HDAC5 antibody (ab1439) at 1/1000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 100 µg

    Secondary
    Goat Anti-Rabbit HRP at 1/10000 dilution

    Predicted band size : 121.9 kDa
  • Formalin-fixed, paraffin-embedded human brain cortex tissue stained for HDAC5 using ab1439 at 1/25 dilution in immunohistochemical analysis.

    See Abreview

  • All lanes : Anti-HDAC5 antibody (ab1439) at 1/1000 dilution

    Lane 1 : Rat hippocampus cell lysate
    Lane 2 : Rat hippocampus cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    Polyclonal anti-rabbit IgG HRP conjugate at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 121.9 kDa


    Exposure time : 10 minutes

    This image is courtesy of an Abreview submitted by Seungyeon Ko

    See Abreview

  • All lanes : Anti-HDAC5 antibody (ab1439)

    Lane 1 : Cell lysate prepared from HeLa cells
    Lane 2 : Cell lysate prepared from HepG2 cells


    Predicted band size : 121.9 kDa
    Observed band size : 124 kDa (why is the actual band size different from the predicted?)
    Western blot analysis of  HDAC5 in HeLa  (Lane1) and HepG2 (Lane2) cell lysates. A band of approximately 124 KDa can be detected.

文献

This product has been referenced in:
  • Mondello P  et al. Panobinostat acts synergistically with ibrutinib in diffuse large B cell lymphoma cells with MyD88 L265 mutations. JCI Insight 2:e90196 (2017). WB ; Human . Read more (PubMed: 28352655) »
  • Liu PK & Liu CH Epigenetics of amphetamine-induced sensitization: HDAC5 expression and microRNA in neural remodeling. J Biomed Sci 23:90 (2016). IHC ; Mouse . Read more (PubMed: 27931227) »

See all 9 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain cortex)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, 100C, 20 min
Permeabilization
No
Specification
brain cortex
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 30 2016

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (7.5%)
Sample
Pig Tissue lysate - whole (Heart)
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 08 2014

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (9% gel)
Sample
Rat Cell lysate - whole cell (hippocampus)
Specification
hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 16 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (9% gel)
Sample
Mouse Tissue lysate - whole (hippocampus)
Specification
hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Hippocampus)
Loading amount
15 µg
Specification
Hippocampus
Gel Running Conditions
Reduced Denaturing (10% SDS gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Seungyeon Ko

Verified customer

提交于 May 14 2013

Application
Western blot
Sample
Rabbit Cell lysate - whole cell (hippocampus)
Loading amount
25 µg
Specification
hippocampus
Gel Running Conditions
Reduced Denaturing (10%SDS)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Miss. Sung-Eun WANG

Verified customer

提交于 Apr 17 2013

Thank you for contacting us.

SInce this is a polyclonal antibody and not a monoclonal, it will bind to many places on the protein and not to one particular site. Polyclonal antibodies are generally not epitope mapped, and not all of are monocl...

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Thank you for inquiry.

I can confirm that tghe immunogen for ab1439 was a synthetic peptide surrounding amino acid 575 of human HDAC-5.

I also included this information on the datasheet for your information.

I hope this in...

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Thank you for your  reply. I am sending a free of charge vial of ab13939 on the order ***. This product is in stock and will arrive on Monday. Please let me know if you have any questions or if there is anything else that we can do for you.

Thanks for letting me know and keeping me updated. I am sorry to hear that this replacement antibody did not work either. I would be happy to send a different antibody to try or issue a credit or refund. I am running into a meeting but I can gi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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