使用敲除细胞株进行验证

Anti-HDAC4抗体[HDAC-144] - ChIP Grade (ab12171)

概述

  • 产品名称Anti-HDAC4抗体[HDAC-144] - ChIP Grade
    参阅全部 HDAC4 一抗
  • 描述
    小鼠单克隆抗体[HDAC-144] to HDAC4 - ChIP Grade
  • 经测试应用适用于: ELISA, ICC, IP, WB, ChIPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken
  • 免疫原

    Synthetic peptide:

    MSSQSHPDGLSGRDQPVEL

    , corresponding to amino acids 1-19 of Human HDAC4 conjugated to KLH with C-terminal added lysine.

  • 阳性对照
    • Total cell extracts of NIH3T3 fibroblast cells.
  • 常规说明Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab12171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 119 kDa).
ChIP Use at an assay dependent concentration. PubMed: 21586557

靶标

  • 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
  • 组织特异性Ubiquitous.
  • 疾病相关Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
  • 序列相似性Belongs to the histone deacetylase family. HD type 2 subfamily.
  • 结构域The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • 翻译后修饰Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
    Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
  • 细胞定位Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AHO3 antibody
    • BDMR antibody
    • EC 3.5.1.98 antibody
    • HA6116 antibody
    • HD 4 antibody
    • HD4 antibody
    • HDAC 4 antibody
    • HDAC A antibody
    • HDAC4 antibody
    • HDAC4_HUMAN antibody
    • HDACA antibody
    • Histone deacetylase 4 antibody
    • Histone Deacetylase A antibody
    • KIAA0288 antibody
    see all

Anti-HDAC4 antibody [HDAC-144] - ChIP Grade 图像



  • Predicted band size : 119 kDa

    Lane 1: Wild-type HAP1 cell lysate (40 µg)
    Lane 2: HDAC4 knockout HAP1 cell lysate (40 µg)
    Lane 3: HeLa cell lysate (40 µg)
    Lane 4: NIH3T3 cell lysate (40 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab12171 observed at 140 kDa. Red - loading control, ab176560, observed at 52 kDa.

    ab12171 was shown to recognize HDAC4  when HDAC4  knockout samples were used, along with additional cross-reactive bands. Wild-type and HDAC4  knockout samples were subjected to SDS-PAGE. Ab12171 and ab176560 (loading control to alpha tubulin) were diluted at 1 µg/ml  and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Mouse IgG (H + L) and IRDye® 680 Goat anti-Rabbit IgG (H + L) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.

Anti-HDAC4 antibody [HDAC-144] - ChIP Grade (ab12171)参考文献

This product has been referenced in:
  • Matsuda KI  et al. Histone Deacetylation during Brain Development Is Essential for Permanent Masculinization of Sexual Behavior. Endocrinology 152:2760-7 (2011). WB, ChIP ; Rat . Read more (PubMed: 21586557) »
  • Kozhemyakina E  et al. PTHrP represses chondrocyte hypertrophy through a PP2A/HDAC4/MEF2 pathway. Mol Cell Biol : (2009). Read more (PubMed: 19704004) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Heart)
Gel Running Conditions Reduced Denaturing (7,5%)
Loading amount 40 µg
Specification Heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

提交于 Jul 02 2015

Thank you for your earlier call and for your patience while I have looked into your enquiry. I apologize that it has taken so long to get back to you. I have asked the lab if the blocking peptide for ab12171 is available for sale, and unfortuna...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Mouse brain)
Loading amount 20 µg
Specification Mouse brain
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (lymphoblastoid cell line GM15850 from Coriell)
Specification lymphoblastoid cell line GM15850 from Coriell
Gel Running Conditions Non-reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"