- 数据表
- 具体的参考文献 (5)
- 实验方案
概述
- 产品名称Anti-HDAC4抗体
参阅全部 HDAC4 一抗 - 描述兔多克隆抗体to HDAC4
- 经测试应用适用于: ICC/IF, WB, IP, IHC-P, Electron Microscopymore details
- 种属反应性与反应: Mouse, Rat, Rabbit, Human
预测可用于: Chicken
- 免疫原
Synthetic peptide:
MSSQSHPDGLSGRDQPVEL
, corresponding to amino acids 1-19 of Human HDAC4 conjugated to KLH with C-terminal added lysine. The corresponding sequence is identical in mouse and chicken. - 阳性对照
- Whole extracts of rat brain, whole extracts of mouse NIH-3T3 cell (Western blotting) and HeLa nuclei (immunoprecipitation).
- 常规说明Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- 存储溶液Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4 -
Concentration information loading... - 纯度Immunogen affinity purified
- 纯化说明The antibody is affinity-purified using the immunizing peptide immobilized on agarose.
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab12172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/250. | |
| WB | 1/500 - 1/1000. Predicted molecular weight: 140 kDa. Additional weak bands may be detected when blotting using different extract preparations. | |
| IP | Use at an assay dependent concentration. 10-20 µg of antibody immunoprecipitates HDAC4 from a RIPA extract of HeLa nuclei from 1 x 107 cells. | |
| IHC-P | Use at an assay dependent concentration. PubMed: 21953612 | |
| Electron Microscopy | Use at an assay dependent concentration. PubMed: 18250163 |
靶标
- 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
- 组织特异性Ubiquitous.
- 疾病相关Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
- 序列相似性Belongs to the histone deacetylase family. HD type 2 subfamily.
- 结构域The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
- 翻译后修饰Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4. - 细胞定位Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
- Information by UniProt
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数据库链接
- Entrez Gene: 9759 Human
- Entrez Gene: 208727 Mouse
- Entrez Gene: 363287 Rat
- Omim: 605314 Human
- SwissProt: P56524 Human
- SwissProt: Q6NZM9 Mouse
- SwissProt: Q99P99 Rat
- Unigene: 20516 Human
see all -
别名
- AHO3 antibody
- BDMR antibody
- EC 3.5.1.98 antibody
see all
Anti-HDAC4 antibody 图像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 antibody (ab12172)This image is courtesy of an anonymous abreview.ab12172 staining HDAC4 in human rhabdomyosarcoma (soft tissue sarcoma) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% H2O2 for 10 minutes at 25°C; antigen retrieval was enzymatic using a proteinase K buffer for 5 minutes at 37oC. Samples were incubated with primary antibody (1/200) for 25 minutes at 25°C. An undiluted HRP-conjugated goat anti-mouse/rabbit IgG polyclonal was used as the secondary antibody.
Anti-HDAC4 antibody (ab12172)参考文献
This product has been referenced in:
- Rönn T et al. A six months exercise intervention influences the genome-wide DNA methylation pattern in human adipose tissue. PLoS Genet 9:e1003572 (2013). WB ; Human . Read more (PubMed: 23825961) »
- Lenoir O et al. Specific control of pancreatic endocrine ß- and d-cell mass by class IIa histone deacetylases HDAC4, HDAC5, and HDAC9. Diabetes 60:2861-71 (2011). IHC-P ; Rat . Read more (PubMed: 21953612) »
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