Synthetic peptide:
MSSQSHPDGLSGRDQPVEL
, corresponding to amino acids 1-19 of Human HDAC4 conjugated to KLH with C-terminal added lysine. The corresponding sequence is identical in mouse and chicken.Our Abpromise guarantee covers the use of ab12172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | 1/250. | |
WB | 1/500 - 1/1000. Predicted molecular weight: 140 kDa. Additional weak bands may be detected when blotting using different extract preparations. | |
IP | Use at an assay dependent concentration. 10-20 µg of antibody immunoprecipitates HDAC4 from a RIPA extract of HeLa nuclei from 1 x 107 cells. | |
IHC-P | Use at an assay dependent concentration. PubMed: 21953612 | |
Electron Microscopy | Use at an assay dependent concentration. PubMed: 18250163 |
ab12172 staining HDAC4 in human rhabdomyosarcoma (soft tissue sarcoma) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% H2O2 for 10 minutes at 25°C; antigen retrieval was enzymatic using a proteinase K buffer for 5 minutes at 37oC. Samples were incubated with primary antibody (1/200) for 25 minutes at 25°C. An undiluted HRP-conjugated goat anti-mouse/rabbit IgG polyclonal was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"