Anti-HDAC3 (phospho S424)抗体(ab61056)

概述

  • 产品名称
    Anti-HDAC3 (phospho S424)抗体
    参阅全部 HDAC3 一抗
  • 描述
    兔多克隆抗体to HDAC3 (phospho S424)
  • 特异性
    ab61056 detects endogenous levels of HDAC3 only when phosphorylated at serine 424.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide derived from human HDAC3 around the phosphorylation site of serine 424 (KESpDV).

  • 阳性对照
    • NIH/3T3 cell extract.

性能

应用

Our Abpromise guarantee covers the use of ab61056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
ELISA 1/5000.

靶标

  • 功能
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
  • 组织特异性
    Widely expressed.
  • 序列相似性
    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • 翻译后修饰
    Sumoylated in vitro.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HD3 antibody
    • HDAC 3 antibody
    • HDAC3 antibody
    • HDAC3_HUMAN antibody
    • Histone deacetylase 3 antibody
    • RPD3 2 antibody
    • RPD3 antibody
    • RPD3-2 antibody
    • SMAP45 antibody
    see all

图片

  • All lanes : Anti-HDAC3 (phospho S424) antibody (ab61056) at 1/500 dilution

    Lane 1 : NIH/3T3 cell extract with no phosphopeptide
    Lane 2 : NIH/3T3 cell extract with phosphopeptide


    Predicted band size : 49 kDa
    Observed band size : 49 kDa

实验方案

文献

ab61056 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (lymphoblastoid cell line)
Loading amount
50 µg
Specification
lymphoblastoid cell line
Gel Running Conditions
Non-reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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提交于 Apr 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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