概述

  • 产品名称Anti-HDAC3抗体[EP462Y]
    参阅全部 HDAC3 一抗
  • 描述
    兔单克隆抗体[EP462Y] to HDAC3
  • 经测试应用适用于: WB, IHC-P, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of Histone deacetylase 3.

  • 阳性对照
    • HeLa nuclear lysate Human breast carcinoma tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

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应用

Our Abpromise guarantee covers the use of ab76295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
    • 组织特异性Widely expressed.
    • 序列相似性Belongs to the histone deacetylase family. HD type 1 subfamily.
    • 翻译后修饰Sumoylated in vitro.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • HD3 antibody
      • HDAC 3 antibody
      • HDAC3 antibody
      • HDAC3_HUMAN antibody
      • Histone deacetylase 3 antibody
      • RPD3 2 antibody
      • RPD3 antibody
      • RPD3-2 antibody
      • SMAP45 antibody
      see all

    Anti-HDAC3 antibody [EP462Y] 图像

    • Anti-HDAC3 antibody [EP462Y] (ab76295) at 1/2000 dilution + HeLa cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 49 kDa
      Observed band size : 49 kDa
    • ab76295, at a 1/100-1/250 dilution, staining human HDAC3 in breast carcinoma, by Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

    Anti-HDAC3 antibody [EP462Y] (ab76295)参考文献

    This product has been referenced in:
    • Choi HK  et al. PINK1 positively regulates HDAC3 to suppress dopaminergic neuronal cell death. Hum Mol Genet 24:1127-41 (2015). Read more (PubMed: 25305081) »
    • Sleiman SF  et al. Histone Deacetylase Inhibitors and Mithramycin A Impact a Similar Neuroprotective Pathway at a Crossroad between Cancer and Neurodegeneration. Pharmaceuticals (Basel) 4:1183-1195 (2011). WB ; Rat . Read more (PubMed: 22582024) »

    See all 2 Publications for this product

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