概述

  • 产品名称Anti-HDAC3抗体- ChIP Grade
    参阅全部 HDAC3 一抗
  • 描述
    兔多克隆抗体to HDAC3 - ChIP Grade
  • 经测试应用适用于: IP, ChIP, WBmore details
  • 种属反应性
    与反应: Human, Zebrafish
    预测可用于: Mouse, Rat, Chicken, Xenopus laevis
  • 免疫原

    Synthetic peptide:

    AKTVAYFYDPDVGNFH

    , corresponding to N terminal amino acids 2-17 of Human HDAC3

  • 阳性对照
    • HeLa cell lysate.

应用

Our Abpromise guarantee covers the use of ab47237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution.
ChIP Use at an assay dependent dilution. Refer to Caretti et al (2003, Fig.3) and Imbriano (2005, Fig 7) for details.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).

靶标

Anti-HDAC3 antibody - ChIP Grade 图像

  • Anti-HDAC3 antibody - ChIP Grade (ab47237) at 2 µg/ml + HeLa cell lysate

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
  • All lanes : Anti-HDAC3 antibody - ChIP Grade (ab47237) at 2 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

Anti-HDAC3 antibody - ChIP Grade (ab47237)参考文献

This product has been referenced in:
  • Doyle K & Fitzpatrick FA Redox signaling, alkylation (carbonylation) of conserved cysteines inactivates class I histone deacetylases 1, 2, and 3 and antagonizes their transcriptional repressor function. J Biol Chem 285:17417-24 (2010). WB . Read more (PubMed: 20385560) »
  • Imbriano C  et al. Direct p53 transcriptional repression: in vivo analysis of CCAAT-containing G2/M promoters. Mol Cell Biol 25:3737-51 (2005). Read more (PubMed: 15831478) »

See all 2 Publications for this product

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