概述

  • 产品名称Anti-HDAC3抗体
    参阅全部 HDAC3 一抗
  • 描述
    兔多克隆抗体to HDAC3
  • 经测试应用适用于: Sandwich ELISA, ELISA, ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthesized non-phosphopeptide derived from human HDAC3 around the phosphorylation site of serine 424.

  • 阳性对照
    • Human skin tissue. Extracts from NIH/3T3 cells. HeLa cells, treated with TNF-a.

性能

应用

Our Abpromise guarantee covers the use of ab63353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [7G6C5] to HDAC3 (ab76359) as Capture.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).

靶标

  • 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the histone deacetylase family. HD type 1 subfamily.
  • 翻译后修饰Sumoylated in vitro.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HD3 antibody
    • HDAC 3 antibody
    • HDAC3 antibody
    • HDAC3_HUMAN antibody
    • Histone deacetylase 3 antibody
    • RPD3 2 antibody
    • RPD3 antibody
    • RPD3-2 antibody
    • SMAP45 antibody
    see all

Anti-HDAC3 antibody 图像

  • Standard Curve for HDAC3 (Analyte: HDAC3 protein (Tagged) (ab82071)); dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [7G6C5] to HDAC3 (ab76359) at 5 ug/ml and Detector Antibody Rabbit polyclonal to HDAC3 (ab63353) at 0.5 ug/ml.
  • All lanes : Anti-HDAC3 antibody (ab63353) at 1/500 dilution

    Lane 1 : extracts from NIH/3T3 cells, without immunizing peptide
    Lane 2 : extracts from NIH/3T3 cells, with immunizing peptide


    Predicted band size : 49 kDa
    Observed band size : 49 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical analysis of HDAC3 expression in paraffin-embedded human skin tissue, using ab63353 at 1/50 dilution. Right: sample treated with immunising peptide. Left: untreated sample.
  • Immunofluorescence analysis of HDAC3 expression in HeLa cells, using ab63353 at 1/500 dilution. Left: Cells treated with TNF-a (20nM, 15mins). Right: Untreated cells.

实验方案

Anti-HDAC3 antibody (ab63353)参考文献

This product has been referenced in:
  • Razidlo DF  et al. Histone deacetylase 3 depletion in osteo/chondroprogenitor cells decreases bone density and increases marrow fat. PLoS One 5:e11492 (2010). WB ; Mouse . Read more (PubMed: 20628553) »

See 1 Publication for this product

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