Anti-HDAC2 (phospho S394)抗体(ab75602)


  • 产品名称Anti-HDAC2 (phospho S394)抗体
    参阅全部 HDAC2 一抗
  • 描述
    兔多克隆抗体to HDAC2 (phospho S394)
  • 特异性ab75602 detects endogenous levels of HDAC2 only when phosphorylated at serine 394.
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from human HDAC2 around the phosphorylation site of serine 394 (E-D-SP-G-D).

  • 阳性对照
    • Extracts from HT-29 cells treated with UV (20min). Human breast carcinoma tissue.



Our Abpromise guarantee covers the use of ab75602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/200.
WB 1/500 - 1/1000. Predicted molecular weight: 55 kDa.
IHC-P 1/50 - 1/100.


  • 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
    Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
  • 组织特异性Widely expressed; lower levels in brain and lung.
  • 序列相似性Belongs to the histone deacetylase family. HD type 1 subfamily.
  • 翻译后修饰S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • D10Wsu179e antibody
    • HD 2 antibody
    • HD2 antibody
    • HDAC 2 antibody
    • Hdac2 antibody
    • HDAC2_HUMAN antibody
    • Histone deacetylase 2 (HD2) antibody
    • Histone deacetylase 2 antibody
    • OTTHUMP00000017046 antibody
    • OTTHUMP00000227077 antibody
    • OTTHUMP00000227078 antibody
    • RPD3 antibody
    • transcriptional regulator homolog RPD3 antibody
    • YAF1 antibody
    • YY1 associated factor 1 antibody
    • YY1 transcription factor binding protein antibody
    • Yy1bp antibody
    see all

Anti-HDAC2 (phospho S394) antibody 图像

  • All lanes : Anti-HDAC2 (phospho S394) antibody (ab75602) at 1/500 dilution

    Lane 1 : Extracts from HT-29 cells (untreated)
    Lane 2 : Extracts from HT-29 cells treated with UV (20min)

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab75602, at 1/50 dilution, in the absence (left panel) and presence (right panel) of the immunising phosphopeptide.
  • ab75602 at 1/100 dilution, staining HDAC2 (phospho S394) in human Hela cells by ICC/IF.

Anti-HDAC2 (phospho S394) antibody (ab75602)参考文献

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lungs)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization Yes - 0.05% tween 20
Specification Lungs
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Formaldehyde

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提交于 May 29 2015